FastQCFastQC Report
Thu 26 May 2016
SRR1512017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159186
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33320.1543174140625217No Hit
CTGTAGGACGTGGAATATGGCAAGA28730.13305940294166413No Hit
GTCCTACAGTGGACATTTCTAAATT27860.1290301067161421No Hit
GTCCTAAAGTGTGTATTTCTCATTT27300.12643653673189803No Hit
CTTTAGGACGTGAAATATGGCGAGG25160.11652539429210823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG705.3010026E-613.9916181
GGTATCA9250.011.8588411
TAGGACC6350.011.6280174
TAGTACC1303.4428012E-810.9227694
AGAACCG700.001536554310.8187425
GTCCTAG3400.010.6583211
GTATCAA19600.010.5436831
CCAACGA909.861508E-510.51870819
CGGTAGG1501.522676E-810.4470751
GGCGAGG8000.010.29518519
GTAGGAC19500.010.1945833
TGTAGGA19650.09.918742
CTGTAGG19400.09.6931621
TGGCGAG16950.09.66231818
CCTACAG17250.09.4938373
GCGCCAC1201.7731512E-59.46661813
GTCCTAC14450.09.4213451
AGGACGT18800.09.3656935
GGACGTG18500.09.3640596
GACGTGG10600.09.2877877
Produced by FastQC (version 0.11.5)