FastQCFastQC Report
Thu 26 May 2016
SRR1512017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159186
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41900.19405461132111823No Hit
GTCCTACAGTGGACATTTCTAAATT33640.1557994540535183No Hit
CTGTAGGACGTGGAATATGGCAAGA33030.15297431532068104No Hit
CTTTAGGACGTGAAATATGGCGAGG30190.13982121040058615No Hit
GTCCTAAAGTGTGTATTTCTCATTT29620.13718132666662344No Hit
GGTATCAACGCAGAGTACTTTTTTT25980.12032312176903705No Hit
TATCAACGCAGAGTACTTTTTTTTT25410.11768323803507434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG500.001505785713.2930629
AAGTCGT1352.7284841E-1112.6600597
GGTATCA10450.012.244651
GAACCGT802.867929E-511.8748546
AGTCGTC1501.7826096E-1011.39405258
TAGGACC6550.011.3172974
CCATGCG600.005899782811.0775529
GGCGAGG7950.010.98899419
TAGAAAT6900.010.8809654
TACACCG700.001487065710.8612885
TCGTCAA1408.42374E-910.8514810
TGGCGAG16450.010.79425918
GACGTGG8050.010.7335297
CAAGTCG1252.2415043E-710.6398716
GGACGTG16750.010.4356946
GTAGGAC18500.010.2741923
CCCCTAG750.002537615610.18561
TGTAGGA19800.010.1756012
AGGACGT17600.010.1516025
GTATTAG1701.4260877E-910.1107051
Produced by FastQC (version 0.11.5)