FastQCFastQC Report
Thu 26 May 2016
SRR1512016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385639
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28780.12063853751552518No Hit
CTGTAGGACGTGGAATATGGCAAGA25230.10575782840572273No Hit
GTCCTACAGTGGACATTTCTAAATT24930.1045003036922183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9750.012.2732051
CGGTAGG802.0670752E-512.2606291
GTCCTAT2100.011.2097191
TAACGCT600.00604194411.0422424
TAGGACC5150.010.8431434
GTATCAA20150.010.270941
CGAAATC1401.0232361E-710.14104713
GGCGAGG5950.010.0221619
TGGACCG951.7074571E-49.9629245
GTAGGAC15500.09.9532823
AAGGCGT1554.3060936E-89.7700936
GTCCTAA9500.09.7052561
AGGACGT15100.09.652825
TGTAGGA15700.09.4961312
GACCTAC1157.839354E-59.3820471
CTGTAGG16650.09.3666781
TAGGACG16100.09.3472034
TATACTG4100.09.233935
TGGCGAG14450.09.17060418
ATAGGAC2800.09.1267513
Produced by FastQC (version 0.11.5)