FastQCFastQC Report
Thu 26 May 2016
SRR1512016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385639
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38400.1609631633285673No Hit
GTCCTACAGTGGACATTTCTAAATT29590.12403385424198715No Hit
CTGTAGGACGTGGAATATGGCAAGA29430.1233631743947848No Hit
GTCCTAAAGTGTGTATTTCTCATTT26260.11007532992208795No Hit
CTTTAGGACGTGAAATATGGCGAGG24560.10294935654556285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.012.4182591
CGATTAC550.00308093612.08478714
GGCGAGG7300.010.92711719
TAGGACC5350.010.4807764
GTATTAG2100.010.45515251
TATACTG3550.010.4407455
GTCCTAA9150.010.3284691
AGGACCT10950.010.241495
TGGCGAG15800.010.21677218
GGACGTG15700.010.1054866
TTAGGAC11250.010.0528613
AGGACGT16150.010.0039535
GTATCAA22550.09.9481691
GTGCTAG2550.09.7331871
GTAGGAC16800.09.7300353
TCTATAC2650.09.6830713
TAGGACG17450.09.5854494
ATGGCGA17600.09.5491417
TAGGACA7500.09.5037564
AGGACGG1002.745307E-49.5037555
Produced by FastQC (version 0.11.5)