FastQCFastQC Report
Thu 26 May 2016
SRR1512015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729213
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32760.18945034533050584No Hit
TATCAACGCAGAGTACTTTTTTTTT19270.11143797785466568No Hit
GGTATCAACGCAGAGTACTTTTTTT19250.11132231830318184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC400.004269403414.7130531
GGTATCA8550.013.4224341
TAGGACT2400.011.8311954
ATAGGAC1451.03682396E-1011.7495993
TGGTATC2600.010.9404752
GTCTTAG1802.0008883E-1110.8985571
GTGGTAT2800.010.8596341
GTATCAA19050.010.6582741
GTCTTAA1406.064329E-810.5093241
TTAGGAC2450.010.4307673
TAGGACA2550.010.3928924
TAGGACG2250.010.0959514
GTATTAG1201.163723E-59.8087021
GGGTATA1104.815275E-59.8087021
GTCTAGA2103.45608E-119.80870151
GTAGGAC3300.09.7517713
AGGCGTG1304.441903E-69.4649547
ATAACCC1002.8594027E-49.4649543
GCCGTGT900.00115192519.4649548
ATAAGAC1951.7971615E-99.2222633
Produced by FastQC (version 0.11.5)