FastQCFastQC Report
Thu 26 May 2016
SRR1512015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729213
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43350.2506920778411913No Hit
TATCAACGCAGAGTACTTTTTTTTT26270.15191882087400452No Hit
GGTATCAACGCAGAGTACTTTTTTT26130.15110920401361774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCG400.005297525314.2426497
GGTATCA9150.012.6253581
CGTGCGC658.059035E-411.686275510
GTGGTAT3900.011.2608871
GTATCAA22300.011.1741641
TGGTATC3500.011.1328092
GTACTAA1407.727067E-910.9111711
GTTCTAG1751.8189894E-1110.911171
ACACCGC803.770976E-410.6875496
GTATTGG1701.2732926E-1010.6704831
CTTAGAC1409.633004E-810.1824473
GCTAGAC951.6377599E-410.0038083
TAGACAG2500.09.5036175
ACTAACC800.004501729.5036173
AGCTCGA900.0011189159.49509910
CTACACT2750.09.3308254
TTAGACT1554.235335E-79.1970494
GTCCTAA2106.0754246E-109.0926421
CTTACAC2304.1836756E-119.0904173
GTAGGAC3600.08.9756393
Produced by FastQC (version 0.11.5)