FastQCFastQC Report
Thu 26 May 2016
SRR1512014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343127
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38290.16341410431444817No Hit
CTGTAGGACGTGGAATATGGCAAGA31180.13307003845715576No Hit
GTCCTACAGTGGACATTTCTAAATT30070.12833277923048986No Hit
GTCCTAAAGTGTGTATTTCTCATTT26720.11403564552839006No Hit
CTTTAGGACGTGAAATATGGCGAGG25480.10874357215806058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7150.012.7097464
GGTATCA10600.011.7382431
ACCGTTC1052.851084E-711.719958
GTCTTAA2450.011.1968851
GGACGTT600.006035554711.04379811
CGGTAGG1251.4653779E-710.9729471
GTAGGAC19600.010.8184163
GAGCGCC700.001537024910.8184169
AGGACGT17700.010.8031355
CGTTATA650.00847217910.5509111
GGACGTG17700.010.535736
TAGAACA6600.010.4700954
TATACTG3800.010.4625475
TGTAGGA19050.010.3372812
GGCGAGG9450.010.31778319
CTGTAGG19150.010.2833011
GGACCTG13250.010.2162586
GACGTGA9700.010.1492357
GTAGAAC4600.010.0834683
TAGGACG19100.010.0608434
Produced by FastQC (version 0.11.5)