FastQCFastQC Report
Thu 26 May 2016
SRR1512014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343127
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47080.20092807602831603No Hit
GTCCTACAGTGGACATTTCTAAATT35760.15261656751853397No Hit
CTGTAGGACGTGGAATATGGCAAGA33030.14096547050159894No Hit
GTCCTAAAGTGTGTATTTCTCATTT30410.12978383160622536No Hit
TATCAACGCAGAGTACTTTTTTTTT29690.12671101481055017No Hit
CTTTAGGACGTGAAATATGGCGAGG29530.12602816663373348No Hit
GGTATCAACGCAGAGTACTTTTTTT28220.12043734718604668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8250.013.0189984
TTAGACA3000.012.673364
CACGACA604.0752013E-412.673364
GAGTACG550.003082156912.0841347
GGTATCA11350.012.0291961
GGCGAGG7500.011.39555619
GTCCTAT2800.010.570611
TTAGGAC11900.010.5433843
AGGACGT17850.010.4901345
GTCCTAA11650.010.490121
AGGACCT14850.010.433125
GGACGTG17550.010.3931046
GGCGAAA1106.076487E-610.35959719
TGTAGGA20250.010.3264422
GTAGGAC19550.010.2586153
CTGTAGG19750.010.2486151
GACGTGA9400.010.2017267
GTATCAA22500.010.0568561
GTATAAC1909.094947E-1110.0501561
CTTAGAC2201.8189894E-129.9370663
Produced by FastQC (version 0.11.5)