FastQCFastQC Report
Thu 26 May 2016
SRR1512013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206699
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32670.1480491902157929No Hit
GTCCTACAGTGGACATTTCTAAATT27490.1245752139281343No Hit
CTGTAGGACGTGGAATATGGCAAGA27220.12335166690155748No Hit
GTCCTAAAGTGTGTATTTCTCATTT25270.11451493837628059No Hit
CTTTAGGACGTGAAATATGGCGAGG24490.11098024696616982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG250.006162913518.9299628
CGGTAGG902.3965185E-814.16628551
TAGGACC6450.012.7667194
GGTATCA10150.011.78823951
GACGTGA8800.011.5085567
AGGACGT17100.011.2362065
GTAGGAC18300.011.1717813
GGACGTG16850.011.0658836
CGTGCGC600.006040739411.04247810
TAGGACG18200.010.9731374
GGCGAGG8150.010.9171619
AGGACCT13700.010.6394675
TGTAGGA17900.010.574492
TGGCGAG18350.010.41968818
TTAGGAC10400.010.1930563
AGGCGTG1304.042813E-710.1930567
ACGTGAA10650.010.1315298
ACCGTCC856.8229885E-410.0217448
CTGTAGG17750.010.0008141
CCAACGA951.7061766E-49.9635919
Produced by FastQC (version 0.11.5)