##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2206699 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.0846277630071 31.0 28.0 34.0 22.0 34.0 2 26.187844377506856 31.0 25.0 34.0 10.0 34.0 3 28.13134460114406 31.0 27.0 34.0 16.0 34.0 4 32.59176897256943 35.0 32.0 37.0 25.0 37.0 5 33.04229303588754 35.0 35.0 37.0 27.0 37.0 6 33.31467363695728 37.0 35.0 37.0 27.0 37.0 7 33.3719981746491 37.0 35.0 37.0 27.0 37.0 8 33.40350451058345 37.0 35.0 37.0 28.0 37.0 9 34.995449311392264 39.0 35.0 39.0 28.0 39.0 10 34.8977518003135 39.0 35.0 39.0 27.0 39.0 11 34.90008605614087 39.0 35.0 39.0 27.0 39.0 12 34.79690297589295 39.0 35.0 39.0 27.0 39.0 13 34.78876049701387 39.0 35.0 39.0 27.0 39.0 14 35.954631782585665 40.0 36.0 41.0 27.0 41.0 15 35.89388131321943 40.0 36.0 41.0 27.0 41.0 16 35.84044085758864 40.0 36.0 41.0 27.0 41.0 17 35.79835537153006 40.0 36.0 41.0 27.0 41.0 18 35.74269667045664 40.0 36.0 41.0 27.0 41.0 19 35.770045665494024 40.0 36.0 41.0 27.0 41.0 20 35.66683992696784 40.0 36.0 41.0 27.0 41.0 21 35.60606272083324 40.0 36.0 41.0 26.0 41.0 22 35.50212104142885 40.0 36.0 41.0 26.0 41.0 23 35.399803054245275 40.0 36.0 41.0 25.0 41.0 24 35.30423043650267 40.0 36.0 41.0 25.0 41.0 25 35.202801106992844 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3639876273116052 1101 2 4.032413323286914 1101 3 2.271592958033306 1101 4 1.4838131872831681 1101 5 1.1851119186992847 1101 6 0.6507161264487493 1101 7 0.47083251312764673 1101 8 0.47543051137067494 1101 9 0.4049769557984533 1101 10 0.584436687588898 1101 11 0.6257769122144765 1101 12 0.5047653004882733 1101 13 0.6311776925404686 1101 14 0.5584119094094149 1101 15 0.7186402076096314 1101 16 0.7061077755134022 1101 17 0.7145930414558421 1101 18 0.7521399867772516 1101 19 0.6841086658573019 1101 20 0.667734392004526 1101 21 0.7196627733574275 1101 22 0.7119870764349159 1101 23 0.7948701653650048 1101 24 0.6343343025119808 1101 25 0.40718028462673317 1102 1 1.6505657880049789 1102 2 4.240553003768916 1102 3 2.4864237237674587 1102 4 1.7033663292732797 1102 5 1.3800567243484565 1102 6 1.008535562899219 1102 7 0.9485858910261271 1102 8 0.9117768147538854 1102 9 1.0578207459473319 1102 10 0.7782466708690734 1102 11 0.8796083187523536 1102 12 0.9092602913812868 1102 13 1.0388814370860047 1102 14 1.156559576263021 1102 15 1.0541030646683112 1102 16 0.9768573475217437 1102 17 1.0093259294183028 1102 18 1.0763387979758505 1102 19 1.0595533615491846 1102 20 1.2172667315484276 1102 21 1.0961039241104515 1102 22 1.0373559594687691 1102 23 1.118036745924769 1102 24 1.0972497595607749 1102 25 1.1632450342134746 1103 1 1.6093897436947273 1103 2 4.199978785137187 1103 3 2.5800762373141204 1103 4 1.8201215036091511 1103 5 1.4785419601375551 1103 6 1.3889169392112635 1103 7 1.095235546086947 1103 8 1.0015957607722328 1103 9 1.0561212398662931 1103 10 1.0055593318419298 1103 11 1.1190810847133932 1103 12 0.8870324493618895 1103 13 1.0656181322713536 1103 14 1.0269144126830838 1103 15 1.2836845464053184 1103 16 1.0972780745470203 1103 17 1.1558165039595636 1103 18 1.1764024301836713 1103 19 1.1042475422795448 1103 20 1.3020640937402703 1103 21 1.2791671349162073 1103 22 1.2970547835634392 1103 23 1.5619044028449238 1103 24 1.542008614836213 1103 25 1.5400261280024807 1104 1 1.5093432179226731 1104 2 3.979530007629876 1104 3 2.300419677857594 1104 4 1.6467777346759362 1104 5 1.2218191962824747 1104 6 1.123036632832516 1104 7 0.8513120408091197 1104 8 0.8867375966436981 1104 9 0.8791581769383114 1104 10 0.943653662377649 1104 11 0.8639776139188271 1104 12 1.0444532866482774 1104 13 0.9232371245025774 1104 14 0.9272939060286376 1104 15 0.8745847406831189 1104 16 0.8529918457551346 1104 17 0.8940074361986987 1104 18 0.9387323436859063 1104 19 0.92034368433076 1104 20 0.8691273038597203 1104 21 0.8312190102178008 1104 22 0.8134950575049444 1104 23 0.9171772536088341 1104 24 0.9499037020229864 1104 25 1.0189281384670466 1105 1 1.5528367440126232 1105 2 4.204794203731325 1105 3 2.5336260612677997 1105 4 1.688188335039193 1105 5 1.1980408771137476 1105 6 0.9631074246123035 1105 7 0.7781929946577719 1105 8 0.6479256728207972 1105 9 0.688863726374187 1105 10 0.5571210748416107 1105 11 0.6247444593517102 1105 12 0.6676554622179651 1105 13 0.661151197004294 1105 14 0.6470611079218926 1105 15 0.6248650400383582 1105 16 0.5650134049074538 1105 17 0.7635214976224489 1105 18 0.8304921233415783 1105 19 0.9399539185850827 1105 20 0.827218195001727 1105 21 0.8818667585764288 1105 22 0.8489173022404515 1105 23 0.858570790330063 1105 24 0.916610839002935 1105 25 0.9835871917118624 1106 1 0.3369224733369798 1106 2 2.147888186297333 1106 3 1.0824794152437889 1106 4 0.33105819902512934 1106 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17 0.7188526246802525 1107 18 0.5951017879341407 1107 19 0.5791540098077519 1107 20 0.6971550561299011 1107 21 0.7347257167548094 1107 22 0.784710710538775 1107 23 0.7140778838427977 1107 24 0.8725803519723669 1107 25 0.786565663680328 1108 1 1.253527438484845 1108 2 3.5872312885595043 1108 3 2.1014568345377924 1108 4 1.357342226080327 1108 5 1.0061698703969313 1108 6 0.8867787719027334 1108 7 0.7436743203105038 1108 8 0.6595165334646254 1108 9 0.5807793418140719 1108 10 0.6549771401053235 1108 11 0.5859867217547077 1108 12 0.5559801577552008 1108 13 0.5873875458708468 1108 14 0.4977338189882303 1108 15 0.6881732130642391 1108 16 0.7024321400773204 1108 17 0.7607099958146435 1108 18 0.7479762736647473 1108 19 0.7157164849529067 1108 20 0.7554401258680841 1108 21 0.7617063317812338 1108 22 0.8374536011485247 1108 23 0.774405570154741 1108 24 0.6537420042864852 1108 25 0.7721155071399792 1109 1 0.8225182840688738 1109 2 2.903297414843802 1109 3 1.6282887946344609 1109 4 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13 -1.22958787765576 2315 14 -1.4321798054450738 2315 15 -1.6949830742180296 2315 16 -1.5298436890804012 2315 17 -1.430829652638387 2315 18 -1.3615667152798139 2315 19 -1.4916338689801236 2315 20 -1.4096291495634006 2315 21 -1.6151071574416989 2315 22 -1.6016657816558961 2315 23 -1.6335988838339688 2315 24 -1.560764000644717 2315 25 -1.5012690470634737 2316 1 -0.8068746434790768 2316 2 -0.44161099056891473 2316 3 -0.3388135694900747 2316 4 -0.6725539055547074 2316 5 -1.0438196339798225 2316 6 -1.160275328821541 2316 7 -1.3711549994045669 2316 8 -1.4903009039791577 2316 9 -1.3675307096221445 2316 10 -1.6512402525979937 2316 11 -1.4554734416092856 2316 12 -1.5600311070363446 2316 13 -1.64665458677851 2316 14 -1.8524332682694649 2316 15 -1.6161535884673555 2316 16 -1.8085910223075956 2316 17 -1.7978387276264485 2316 18 -1.825259120947706 2316 19 -1.6034071534889591 2316 20 -1.5715377628415368 2316 21 -1.8555032727099743 2316 22 -1.9098243551314553 2316 23 -1.8840558174432687 2316 24 -1.8167983900731457 2316 25 -1.8160106487192635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91347.0 3 3.0 4 837.0 5 3688.0 6 4209.0 7 3896.0 8 3510.0 9 3485.0 10 3269.0 11 3173.0 12 3133.0 13 3058.0 14 3193.0 15 3320.0 16 3400.0 17 3788.0 18 4286.0 19 5380.0 20 6788.0 21 8784.0 22 10652.0 23 12801.0 24 14633.0 25 16575.0 26 18880.0 27 21289.0 28 24171.0 29 29417.0 30 36713.0 31 46250.0 32 59029.0 33 77894.0 34 106518.0 35 153974.0 36 246288.0 37 341408.0 38 827660.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.394544974189955 19.13482536585189 13.135955560771995 25.33467409918616 2 16.889563794031222 21.699859415631064 39.185427683569664 22.225149106768054 3 18.1743409499891 24.83800463950906 30.067897796663708 26.919756613838135 4 12.02452169507486 17.31454992275793 39.22138905215437 31.43953933001284 5 13.077451886279007 37.730293075766106 36.269241976363794 12.92301306159109 6 30.907610291585247 37.95615707827449 18.60591581259947 12.530316817540793 7 27.50171183292329 31.73953493430685 23.04269861906857 17.716054613701278 8 24.515860348810754 37.264274495195984 21.115259088466114 17.104606067527136 9 26.01551004464134 15.817472160906403 20.835102567228244 37.33191522722401 10 14.432475323979684 29.385593037920476 35.05424623317111 21.127685404928723 11 33.68792028273906 22.85259566438377 25.087744182600346 18.371739870276826 12 22.77999854080688 24.945993993743595 32.31863521033 19.955372255119524 13 28.433964034061738 22.264477393609187 26.16423898320523 23.137319589123845 14 20.649395318527812 22.280519454624308 28.281111288852717 28.788973937995166 15 22.642689374491038 30.9947573275739 23.749093102412246 22.613460195522812 16 21.891204917390183 28.566061796375493 27.826359643975007 21.71637364225932 17 21.463597889879864 28.809955503673134 28.142895791406076 21.583550815040926 18 21.232142954250072 27.929498090354247 29.861711557389974 20.976647398005703 19 23.095380950999868 27.374418132513522 27.90054597659479 21.62965493989182 20 23.185902868096562 27.62830302398615 28.38436753689916 20.80142657101813 21 22.845015888323125 27.15688744759029 27.473611607086212 22.52448505700038 22 22.747346120294107 28.243669774660972 27.748762278088073 21.260221826956847 23 22.09533019620354 27.98524304296756 27.954699531605737 21.96472722922316 24 22.57679910128205 27.23180642217176 28.703325646134793 21.488068830411397 25 22.5456124124311 27.6098012816475 28.11748450508023 21.72710180084117 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 162.0 2 371.0 3 580.0 4 580.0 5 580.0 6 1330.5 7 2081.0 8 2081.0 9 2081.0 10 3367.0 11 4653.0 12 4653.0 13 4653.0 14 8202.5 15 11752.0 16 11752.0 17 11752.0 18 20423.5 19 29095.0 20 29095.0 21 29095.0 22 45604.5 23 62114.0 24 62114.0 25 62114.0 26 86856.5 27 111599.0 28 111599.0 29 111599.0 30 141124.0 31 170649.0 32 170649.0 33 170649.0 34 194748.0 35 218847.0 36 218847.0 37 218847.0 38 237564.5 39 256282.0 40 256282.0 41 256282.0 42 267909.5 43 279537.0 44 279537.0 45 279537.0 46 283230.5 47 286924.0 48 286924.0 49 286924.0 50 275010.5 51 263097.0 52 263097.0 53 263097.0 54 232924.5 55 202752.0 56 202752.0 57 202752.0 58 172596.0 59 142440.0 60 142440.0 61 142440.0 62 114660.5 63 86881.0 64 86881.0 65 86881.0 66 66371.5 67 45862.0 68 45862.0 69 45862.0 70 32987.0 71 20112.0 72 20112.0 73 20112.0 74 13867.5 75 7623.0 76 7623.0 77 7623.0 78 5064.5 79 2506.0 80 2506.0 81 2506.0 82 1642.0 83 778.0 84 778.0 85 778.0 86 514.5 87 251.0 88 251.0 89 251.0 90 158.0 91 65.0 92 65.0 93 65.0 94 56.0 95 47.0 96 47.0 97 47.0 98 28.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.490145235032055 3 0.0 4 0.0 5 0.0 6 1.3594966961964455E-4 7 0.0 8 4.531655653988152E-5 9 0.0 10 9.063311307976304E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.172158957791706E-4 19 4.984821219386966E-4 20 4.078490088589336E-4 21 0.002129878157374431 22 0.0010875973569571564 23 4.984821219386966E-4 24 0.0 25 3.6253245231905216E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2206699.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.93108449325541 #Duplication Level Percentage of deduplicated Percentage of total 1 83.31665364900303 62.43007214268742 2 11.648821512798776 17.457176580447527 3 2.942757321327847 6.615119924626886 4 0.9822885157156113 2.9441577507136363 5 0.4171312554277446 1.5628048672617019 6 0.20598225902518388 0.9260684433076606 7 0.12376172733792547 0.6491520313732531 8 0.07278840036554288 0.43632910223355165 9 0.049156765656713695 0.33150327847545336 >10 0.21549658692429416 2.6938488330463493 >50 0.012684562222217987 0.6413797097858553 >100 0.010461493232553175 1.544008801318542 >500 0.001282877885337676 0.6858463342762543 >1k 7.330730773347175E-4 1.082532200445951 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3267 0.1480491902157929 No Hit GTCCTACAGTGGACATTTCTAAATT 2749 0.1245752139281343 No Hit CTGTAGGACGTGGAATATGGCAAGA 2722 0.12335166690155748 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2527 0.11451493837628059 No Hit CTTTAGGACGTGAAATATGGCGAGG 2449 0.11098024696616982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAACG 25 0.0061629135 18.929962 8 CGGTAGG 90 2.3965185E-8 14.1662855 1 TAGGACC 645 0.0 12.766719 4 GGTATCA 1015 0.0 11.7882395 1 GACGTGA 880 0.0 11.508556 7 AGGACGT 1710 0.0 11.236206 5 GTAGGAC 1830 0.0 11.171781 3 GGACGTG 1685 0.0 11.065883 6 CGTGCGC 60 0.0060407394 11.042478 10 TAGGACG 1820 0.0 10.973137 4 GGCGAGG 815 0.0 10.91716 19 AGGACCT 1370 0.0 10.639467 5 TGTAGGA 1790 0.0 10.57449 2 TGGCGAG 1835 0.0 10.419688 18 TTAGGAC 1040 0.0 10.193056 3 AGGCGTG 130 4.042813E-7 10.193056 7 ACGTGAA 1065 0.0 10.131529 8 ACCGTCC 85 6.8229885E-4 10.021744 8 CTGTAGG 1775 0.0 10.000814 1 CCAACGA 95 1.7061766E-4 9.96359 19 >>END_MODULE