FastQCFastQC Report
Thu 26 May 2016
SRR1512013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206699
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41050.18602446459621363No Hit
GTCCTACAGTGGACATTTCTAAATT33070.14986185247738817No Hit
CTGTAGGACGTGGAATATGGCAAGA32190.1458739955018786No Hit
CTTTAGGACGTGAAATATGGCGAGG29050.1316445967483558No Hit
GTCCTAAAGTGTGTATTTCTCATTT28920.13105548151333735No Hit
TATCAACGCAGAGTACTTTTTTTTT25790.11687139931635443No Hit
GGTATCAACGCAGAGTACTTTTTTT24940.1130194920104645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC350.00217803816.2773638
GGTATCA9550.012.2989971
TAGGACC6200.011.9557784
AATCCCG1552.5465852E-1111.64077219
GGCGTGC1251.8470018E-811.3941558
GGCGAGG7100.011.23520119
GGACGTG16750.010.889156
GCGTGCG1053.4891364E-610.8515759
AGGACGT16950.010.8208795
GTCCTAT1604.984031E-1010.7428751
GTCCTAA10050.010.7369361
TTAGGAC11150.010.6539363
GTCTTAC1803.274181E-1110.6102461
GTAGGAC17000.010.5654463
GTCCTAC16900.010.5097941
GACGTGA8950.010.5029927
TGTAGGA19100.010.4984222
GTATCAA19500.010.3817191
CGTGAAA10000.010.349699
TCGAACT1752.382876E-1010.31039819
Produced by FastQC (version 0.11.5)