FastQCFastQC Report
Thu 26 May 2016
SRR1512012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055672
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38580.18767585490292227No Hit
GTCCTACAGTGGACATTTCTAAATT29750.14472153145054267No Hit
CTGTAGGACGTGGAATATGGCAAGA28230.13732735572601076No Hit
GTCCTAAAGTGTGTATTTCTCATTT27260.1326087041123292No Hit
CTTTAGGACGTGAAATATGGCGAGG24290.11816087391373722No Hit
GGTATCAACGCAGAGTACTTTTTTT23870.11611774641090603No Hit
TATCAACGCAGAGTACTTTTTTTTT23240.11305305515665924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG808.7324224E-814.7108931
TAGGACC4700.013.2912074
GGTATCA10050.013.1739331
CCGTCCA802.9779289E-511.8311889
ATTATAC1407.239578E-1011.4931543
CGTTAGG700.001143479511.2082981
GTGCTCG600.006039554711.0427113
GGACGTA600.00604064811.0424426
GGCGAGG7650.010.764585519
GTAGGAC16250.010.6007443
GTATCAA20250.010.4610791
AATCCCG1002.4979363E-510.41195219
GTACTAA1354.1666863E-710.1704931
TGTAGGA16000.010.1137372
GGTAGGA2251.8189894E-1210.02522
AGGACGT17850.010.0217125
TCCAACG951.7060469E-49.9635918
GACGTGG8500.09.910367
GGACGTG17600.09.8951756
ACGAAAT1252.69482E-69.84354812
Produced by FastQC (version 0.11.5)