##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512012_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2055672 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.27034273950319 31.0 30.0 34.0 23.0 34.0 2 26.330565382025927 31.0 25.0 34.0 10.0 34.0 3 28.298315100852665 31.0 27.0 34.0 16.0 34.0 4 32.79290762339517 35.0 33.0 37.0 26.0 37.0 5 33.24991730198203 35.0 35.0 37.0 27.0 37.0 6 33.52888982289004 37.0 35.0 37.0 28.0 37.0 7 33.59712249814173 37.0 35.0 37.0 28.0 37.0 8 33.63309273074693 37.0 35.0 37.0 28.0 37.0 9 35.237940683144004 39.0 35.0 39.0 29.0 39.0 10 35.147485591086514 39.0 35.0 39.0 29.0 39.0 11 35.148982911670736 39.0 35.0 39.0 29.0 39.0 12 35.05058054008616 39.0 35.0 39.0 28.0 39.0 13 35.04193081386525 39.0 35.0 39.0 28.0 39.0 14 36.22505779132079 40.0 37.0 41.0 28.0 41.0 15 36.16312524566176 40.0 36.0 41.0 27.0 41.0 16 36.10847693600925 40.0 36.0 41.0 27.0 41.0 17 36.0697514000288 40.0 36.0 41.0 27.0 41.0 18 36.01892811693694 40.0 36.0 41.0 27.0 41.0 19 36.04525430126985 40.0 37.0 41.0 27.0 41.0 20 35.946494868831216 40.0 36.0 41.0 27.0 41.0 21 35.883041166100426 40.0 36.0 41.0 27.0 41.0 22 35.78001402947552 40.0 36.0 41.0 27.0 41.0 23 35.68320335150744 40.0 36.0 41.0 27.0 41.0 24 35.593899707735474 40.0 36.0 41.0 27.0 41.0 25 35.48757000143992 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.364944621844721 1101 2 4.1888293761390685 1101 3 2.30368389699278 1101 4 1.483765237781789 1101 5 1.0281458827276708 1101 6 0.7395880310458907 1101 7 0.6709020384719722 1101 8 0.4620826642552913 1101 9 0.5358911603421319 1101 10 0.5324349279141671 1101 11 0.4623800214184257 1101 12 0.4752194115484727 1101 13 0.45386316720756525 1101 14 0.32350783235272473 1101 15 0.5889341532185526 1101 16 0.5402972352646387 1101 17 0.6968111934687471 1101 18 0.6786313297589217 1101 19 0.6155156495023562 1101 20 0.5381734684978383 1101 21 0.662114845526304 1101 22 0.6398991476911178 1101 23 0.7183753780587878 1101 24 0.6601199910900633 1101 25 0.45276844010516726 1102 1 1.7356192190168223 1102 2 4.367380996454418 1102 3 2.68039461001797 1102 4 1.91697990165369 1102 5 1.4701932280612056 1102 6 1.2422405366102112 1102 7 1.1089715258343062 1102 8 1.1627934204984882 1102 9 1.1966405606921953 1102 10 1.0548011501555976 1102 11 1.092522064622031 1102 12 1.1400988332914537 1102 13 1.1302403812303652 1102 14 1.2480473804363612 1102 15 1.2305136635584617 1102 16 1.2452388549317206 1102 17 1.3849554119790284 1102 18 1.4089806393511637 1102 19 1.4031009150410299 1102 20 1.4209770210185297 1102 21 1.3978285910154256 1102 22 1.1729159090772612 1102 23 1.2836426814708446 1102 24 1.2883280281999276 1102 25 1.3858201360520397 1103 1 1.5951215915330756 1103 2 4.303528760418519 1103 3 2.6174561914335293 1103 4 1.8085638249380978 1103 5 1.4880526821860087 1103 6 1.3663677441597386 1103 7 1.0475250305250228 1103 8 0.9785914549964687 1103 9 1.0086270259087726 1103 10 1.0973432094488587 1103 11 1.0352598305044864 1103 12 1.0219216954266486 1103 13 1.020288467154053 1103 14 1.0497157689974372 1103 15 1.2682332628770965 1103 16 1.1562579390687944 1103 17 1.1286595188705348 1103 18 1.1508741569387553 1103 19 1.0908318973528992 1103 20 1.1607571732386148 1103 21 1.190286289039058 1103 22 1.2724084702622918 1103 23 1.3775434194586111 1103 24 1.3042439951303066 1103 25 1.33411823410988 1104 1 1.3752186682974532 1104 2 3.852125127388284 1104 3 2.1937056250785965 1104 4 1.5949243651735827 1104 5 1.1256729867680235 1104 6 0.9406897027887737 1104 7 0.7543373704614211 1104 8 0.7878253999239391 1104 9 0.7981937508923593 1104 10 0.7376872764709645 1104 11 0.6997086830839194 1104 12 0.9649820242321852 1104 13 0.906038520364433 1104 14 0.9940819316808671 1104 15 0.8681404872272722 1104 16 1.0665024798500866 1104 17 1.0389228804877106 1104 18 1.049455315012466 1104 19 1.0428257573319755 1104 20 0.8148861186601266 1104 21 0.9149283264277557 1104 22 0.8793195365539859 1104 23 0.9804381747282349 1104 24 0.9723022965443349 1104 25 1.0864556850546165 1105 1 1.5602659774871093 1105 2 4.368206967868623 1105 3 2.600684287660588 1105 4 1.7220090228592824 1105 5 1.3490782319462085 1105 6 1.1108604787595624 1105 7 1.0158073370818812 1105 8 0.8706174592048797 1105 9 0.9074689479911768 1105 10 0.9018209604107028 1105 11 0.8896350823232879 1105 12 0.9724617318308262 1105 13 0.8873337780296495 1105 14 0.996845967470243 1105 15 0.8792513637667483 1105 16 1.0346961296831125 1105 17 1.2154239630358106 1105 18 0.9832568533332591 1105 19 1.0766642797808714 1105 20 1.04094202333647 1105 21 1.0349031177958352 1105 22 1.1229870797366672 1105 23 1.1197518337564674 1105 24 1.1329392992817873 1105 25 1.2786887053911968 1106 1 0.6358660818586195 1106 2 2.4428119789740066 1106 3 1.314921707393168 1106 4 0.7747005986781872 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0.9675494453979283 1107 17 0.8881085988497901 1107 18 0.7097793843141744 1107 19 0.8354181175311481 1107 20 0.8945663519565628 1107 21 1.100017732325604 1107 22 0.9455153077573542 1107 23 1.0416254527821494 1107 24 1.1414631255713346 1107 25 1.0248784840441019 1108 1 1.2447100268681517 1108 2 3.478276804730953 1108 3 2.163252218577167 1108 4 1.4570479953934452 1108 5 0.9489718940475456 1108 6 0.7443392330494092 1108 7 0.5977293769635281 1108 8 0.5784532595680574 1108 9 0.4891885178800095 1108 10 0.43183707805682303 1108 11 0.49583118144845173 1108 12 0.5525352042170653 1108 13 0.5154119215702906 1108 14 0.49691957154428934 1108 15 0.5156989724809051 1108 16 0.603391070661111 1108 17 0.5697591489343026 1108 18 0.5998936786037703 1108 19 0.7066526018948736 1108 20 0.7513116380697014 1108 21 0.7281232184335309 1108 22 0.8042793227138247 1108 23 0.7950377021034711 1108 24 0.7905745344221131 1108 25 0.7618178520871766 1109 1 0.611821115947361 1109 2 2.695330266614743 1109 3 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-1.5859259946539694 2315 11 -1.6232565838937845 2315 12 -1.6735612624448493 2315 13 -1.7923803495163355 2315 14 -1.919215113420222 2315 15 -2.0395837222076665 2315 16 -1.9684897441001468 2315 17 -1.955115481357808 2315 18 -1.904406789705007 2315 19 -1.8826537657369755 2315 20 -1.8904015951812383 2315 21 -1.8353308752311293 2315 22 -2.2111418423988383 2315 23 -2.0968039196211663 2315 24 -1.9937422861409928 2315 25 -1.8868465440796456 2316 1 -0.3692655608865536 2316 2 0.08027656747568557 2316 3 0.14440120897250353 2316 4 -0.2851658050011281 2316 5 -0.6831264131256702 2316 6 -0.6337682630691006 2316 7 -0.8574857339011288 2316 8 -1.0389268717294229 2316 9 -1.0095119703022846 2316 10 -1.2472449040752807 2316 11 -1.1576091417196608 2316 12 -1.079355243684006 2316 13 -1.3295808964135247 2316 14 -1.415746833283471 2316 15 -1.236386316961827 2316 16 -1.331661342924228 2316 17 -1.5147767821934792 2316 18 -1.3756608534067283 2316 19 -1.4477428302802124 2316 20 -1.4339353283155418 2316 21 -1.4728323428195509 2316 22 -1.4765896925480888 2316 23 -1.5023181192259116 2316 24 -1.3994262343962873 2316 25 -1.471204393899228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75993.0 3 1.0 4 669.0 5 3026.0 6 3580.0 7 3290.0 8 2951.0 9 3058.0 10 2810.0 11 2798.0 12 2738.0 13 2810.0 14 2772.0 15 2792.0 16 3071.0 17 3249.0 18 3727.0 19 4855.0 20 6175.0 21 7804.0 22 9502.0 23 11475.0 24 13087.0 25 14746.0 26 16678.0 27 19033.0 28 21958.0 29 26777.0 30 33338.0 31 42420.0 32 54720.0 33 71888.0 34 99991.0 35 144439.0 36 231819.0 37 321023.0 38 784609.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.277123976976874 19.171005880315537 13.26802135749283 25.28384878521476 2 16.948956904681616 21.75865230542488 38.90776536315665 22.38462542673685 3 18.075889538798016 24.947267852069785 30.097311244206278 26.879531364925924 4 12.237993220708363 17.4557030499029 38.817574009861495 31.488729719527242 5 13.144217559999843 37.756169272140696 35.94114236123273 13.158470806626738 6 30.89403367852459 37.6525535202114 18.633566055285083 12.81984674597893 7 27.51119828455123 31.875124047026958 22.890811374577265 17.722866293844543 8 24.788001198634802 36.72730863678642 21.10857179550045 17.376118369078338 9 26.248545487801557 15.760685556839807 20.80210267007577 37.18866628528287 10 14.898680334216744 29.07832572511568 34.68033810841418 21.342655832253392 11 33.36709358302297 22.928511941593797 25.16724457987461 18.537149895508623 12 23.064671795889616 24.928976996330153 31.894874279554326 20.111476928225905 13 28.345183472849754 22.42896726715157 26.095602800446766 23.130246459551913 14 20.85561315229278 22.3743379293973 28.01351577489016 28.756533143419766 15 22.602243937748824 30.901330562463276 23.823742309084327 22.672683190703573 16 21.972376916161725 28.374371008604488 27.81080833907355 21.842443736160245 17 21.54234722270868 28.668143555975856 28.052481135122726 21.73702808619274 18 21.32779739840246 27.738974344006305 29.902902230913675 21.030326026677564 19 23.24517963157188 27.25184029203386 27.861281967959556 21.641698108434696 20 23.362932939234053 27.466728414940615 28.317453584205154 20.85288506162017 21 22.858178350951786 26.982890723434892 27.42918160546018 22.729749320153143 22 23.032661958627827 28.099081888644516 27.584739104694272 21.283517048033378 23 22.13831252319827 27.888272754735855 27.981917146361074 21.991497575704802 24 22.82735069344788 27.06835241064957 28.537800327873637 21.566496568028914 25 22.59021902412067 27.524780314292606 28.152801235999657 21.732199425587062 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 251.0 1 251.0 2 426.5 3 602.0 4 602.0 5 602.0 6 1332.0 7 2062.0 8 2062.0 9 2062.0 10 3296.0 11 4530.0 12 4530.0 13 4530.0 14 7439.0 15 10348.0 16 10348.0 17 10348.0 18 18223.0 19 26098.0 20 26098.0 21 26098.0 22 40689.0 23 55280.0 24 55280.0 25 55280.0 26 78613.5 27 101947.0 28 101947.0 29 101947.0 30 130378.0 31 158809.0 32 158809.0 33 158809.0 34 180603.0 35 202397.0 36 202397.0 37 202397.0 38 218626.0 39 234855.0 40 234855.0 41 234855.0 42 246467.0 43 258079.0 44 258079.0 45 258079.0 46 262982.0 47 267885.0 48 267885.0 49 267885.0 50 257518.5 51 247152.0 52 247152.0 53 247152.0 54 219612.5 55 192073.0 56 192073.0 57 192073.0 58 163588.5 59 135104.0 60 135104.0 61 135104.0 62 108978.0 63 82852.0 64 82852.0 65 82852.0 66 63673.0 67 44494.0 68 44494.0 69 44494.0 70 32095.0 71 19696.0 72 19696.0 73 19696.0 74 13546.5 75 7397.0 76 7397.0 77 7397.0 78 4987.5 79 2578.0 80 2578.0 81 2578.0 82 1699.0 83 820.0 84 820.0 85 820.0 86 535.5 87 251.0 88 251.0 89 251.0 90 160.0 91 69.0 92 69.0 93 69.0 94 51.0 95 33.0 96 33.0 97 33.0 98 21.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.488932086441806 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.837507150946259E-4 19 9.729178584910432E-4 20 6.810425009437304E-4 21 0.003016045361322234 22 0.0018971898240575345 23 0.0011188555372647 24 9.729178584910432E-5 25 3.891671433964173E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2055672.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.58909742295832 #Duplication Level Percentage of deduplicated Percentage of total 1 84.85169122245877 64.13862754317213 2 10.718280145042037 16.203702441802847 3 2.495050267417423 5.657957932169814 4 0.8360333124931174 2.5278001402752324 5 0.3709241407055751 1.4018910504160411 6 0.1977566695324898 0.8968948895598685 7 0.1206655992478914 0.6384702616003957 8 0.08181320246387468 0.49473489052208286 9 0.053645839205631 0.36495365094457183 >10 0.24695883978682762 3.202619646687568 >50 0.013961915857000302 0.7312702933656563 >100 0.010941099253011805 1.6508010241475106 >500 0.0013015694494096265 0.6701949409353117 >1k 9.761770870571108E-4 1.420081294400978 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3858 0.18767585490292227 No Hit GTCCTACAGTGGACATTTCTAAATT 2975 0.14472153145054267 No Hit CTGTAGGACGTGGAATATGGCAAGA 2823 0.13732735572601076 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2726 0.1326087041123292 No Hit CTTTAGGACGTGAAATATGGCGAGG 2429 0.11816087391373722 No Hit GGTATCAACGCAGAGTACTTTTTTT 2387 0.11611774641090603 No Hit TATCAACGCAGAGTACTTTTTTTTT 2324 0.11305305515665924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.864589292455217E-5 0.0 4 0.0 0.0 0.0 4.864589292455217E-5 0.0 5 0.0 0.0 0.0 4.864589292455217E-5 0.0 6 0.0 0.0 0.0 4.864589292455217E-5 0.0 7 0.0 0.0 0.0 4.864589292455217E-5 0.0 8 0.0 0.0 0.0 4.864589292455217E-5 0.0 9 0.0 0.0 0.0 9.729178584910434E-5 0.0 10 0.0 4.864589292455217E-5 0.0 9.729178584910434E-5 0.0 11 0.0 9.729178584910434E-5 0.0 9.729178584910434E-5 0.0 12 0.0 1.4593767877365649E-4 0.0 9.729178584910434E-5 0.0 13 0.0 1.9458357169820867E-4 0.0 9.729178584910434E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 80 8.7324224E-8 14.710893 1 TAGGACC 470 0.0 13.291207 4 GGTATCA 1005 0.0 13.173933 1 CCGTCCA 80 2.9779289E-5 11.831188 9 ATTATAC 140 7.239578E-10 11.493154 3 CGTTAGG 70 0.0011434795 11.208298 1 GTGCTCG 60 0.0060395547 11.04271 13 GGACGTA 60 0.006040648 11.042442 6 GGCGAGG 765 0.0 10.7645855 19 GTAGGAC 1625 0.0 10.600744 3 GTATCAA 2025 0.0 10.461079 1 AATCCCG 100 2.4979363E-5 10.411952 19 GTACTAA 135 4.1666863E-7 10.170493 1 TGTAGGA 1600 0.0 10.113737 2 GGTAGGA 225 1.8189894E-12 10.0252 2 AGGACGT 1785 0.0 10.021712 5 TCCAACG 95 1.7060469E-4 9.96359 18 GACGTGG 850 0.0 9.91036 7 GGACGTG 1760 0.0 9.895175 6 ACGAAAT 125 2.69482E-6 9.843548 12 >>END_MODULE