FastQCFastQC Report
Thu 26 May 2016
SRR1512012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055672
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49460.24060258640483503No Hit
GTCCTACAGTGGACATTTCTAAATT33410.1625259282609288No Hit
TATCAACGCAGAGTACTTTTTTTTT31540.15342914628403753No Hit
CTGTAGGACGTGGAATATGGCAAGA31410.15279674967601836No Hit
GGTATCAACGCAGAGTACTTTTTTT30200.14691059663214753No Hit
GTCCTAAAGTGTGTATTTCTCATTT29860.14525663627271276No Hit
CTTTAGGACGTGAAATATGGCGAGG29560.14379725948497618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG250.00604983718.9906859
TAGGACC5550.013.0137094
GGTATCA10100.012.1932581
TAAGACG600.005860971811.08733754
GTATCAA22500.010.522541
AGGACCT12150.010.4811535
GTGCGCC1106.0826114E-610.35855511
ATGACCG750.002640267810.1369945
GTCTAAG2450.010.1311481
ATAGGAC2550.010.0624583
TGGCGAG17350.09.85131518
GTCCTAA9600.09.8449931
GGCGAGG6950.09.83809819
ATTACCC1651.0162694E-89.7914153
GTCCTAC18050.09.7317751
GGACCTG13400.09.7117036
GACGTGG9700.09.6911227
TGTAGGA19500.09.6496392
GCCTTAC1409.903106E-79.546661
GTAGGAC18550.09.5290493
Produced by FastQC (version 0.11.5)