FastQCFastQC Report
Thu 26 May 2016
SRR1512011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907202
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27240.14282703143138484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1051.4479156E-913.5222926
CCGACCA707.541894E-613.5222919
CGTCCCA751.5351841E-512.62080610
TAGGACC3000.011.9897664
CGTGCGC907.770359E-611.56907210
GTGGTAT3900.011.5609971
GGTATCA8350.011.2690381
GGACGTG5750.011.0294876
GTCTAAG1905.456968E-1210.8334751
AGGACGT6050.010.7954835
GTCATAT2001.8189894E-1210.7818871
GACGTGA3350.010.73710257
GTATCAA16500.010.6333761
GTAGGAC6800.010.4400053
TAGACAG2001.8189894E-1210.4121655
TTAGGAC5200.010.0116973
TGGTATC3350.09.948012
TATGGCG6300.09.91686716
TGGCGAG6300.09.91686718
TAGGACG6800.09.8832044
Produced by FastQC (version 0.11.5)