FastQCFastQC Report
Thu 26 May 2016
SRR1512011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907202
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30020.1574033584276862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT500.001500794713.2987896
TACACCG655.4342847E-513.158865
GGTATCA9650.013.0607751
ACAACGC604.1104518E-412.66028
CTATACG550.003060136712.0955184
TGCTCGA550.003080643212.08473710
ACGGTCC550.003080643212.0847378
TAGGACC3050.011.2173894
AGTGCGC600.00589886711.0776758
CCGTGTA700.001498039210.85169
GTACTAG1355.281072E-810.6091311
TCAGTAC2100.010.4087283
GTCCTAC6700.010.2607711
TTACACC2053.6379788E-1210.1990084
CGCTCCA856.6254375E-410.05368810
GTATTAA2300.09.9633581
GTATCAA18350.09.7303361
GTAGGAC7350.09.6975733
AGGACGT6600.09.6476155
GTATTAC1606.136179E-89.5482181
Produced by FastQC (version 0.11.5)