FastQCFastQC Report
Thu 26 May 2016
SRR1512010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778637
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25660.1442677735816808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG250.006164180618.9290435
GACAGCG1302.7757778E-911.6483147
GGTATCA7200.011.5837141
TAGGACC3850.011.5540914
AGACCCG752.147464E-411.3574265
ATTAGAC951.4179772E-510.95892053
CCTATAC1900.010.95892053
GTATAAG2350.010.8559261
GGTCTAT1104.1084986E-610.7040951
CCGCTCT803.904901E-410.6472879
CTATACT2450.010.4302894
CGTGCGC1002.5007825E-510.41068110
TAGGAGT1659.913492E-1010.3249334
GTAGGAC7900.010.0635413
CCTAGAC1701.6625563E-910.0212583
TGCACCG951.7073705E-49.9626555
GTATTAA1709.811629E-99.8120871
GTCCTAC6900.09.8120861
GTATCAA14150.09.7774141
GTCCTAA4000.09.5667851
Produced by FastQC (version 0.11.5)