FastQCFastQC Report
Thu 26 May 2016
SRR1512010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778637
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30470.17131095327489532No Hit
TATCAACGCAGAGTACTTTTTTTTT18790.10564269156663221No Hit
GGTATCAACGCAGAGTACTTTTTTT18180.10221309913152599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGTA350.00217836116.2767513
CGTGCGC1001.0153599E-813.29268110
TGCGATT550.003081423512.08425412
GGTATCA9450.011.9253011
TAGGACC3750.011.91216854
TACACCG1157.029848E-811.5705615
GTGCGCC1406.85759E-1011.52936611
GACGTGA3300.011.2210937
ACTAGAT855.2986303E-511.1816363
GCGCCAC1201.2851706E-711.07723413
GCGTGCG1201.2851706E-711.0772349
AAGGCGT1752.0008883E-1110.8566616
TATGTCG700.001498451210.85116716
GTAGGAC7800.010.7229023
GGACGTG6050.010.6772126
GGCGAGG2950.010.62216519
GTCTTAG1907.2759576E-1210.5556471
GTGGTAT3800.010.5556471
AGGACTG4150.010.5349865
TAGGACA3700.010.5318924
Produced by FastQC (version 0.11.5)