FastQCFastQC Report
Thu 26 May 2016
SRR1512009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895929
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28920.1525373576753138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC707.540373E-613.52254510
GGTATCA9100.013.1391241
GTCAACG656.17804E-412.0621462
GCGTGCG550.00315292612.0473589
TAGGACC3300.011.7605164
ACCGTCC752.1449539E-411.3589388
TACGGTG600.006036409611.0434125
GGCGAGG5100.010.7652919
CAGAGCG1159.237865E-710.7004489
GTACCGT803.8993466E-410.6490046
TACGACT803.8993466E-410.6490044
GTATTAG1751.3278623E-1010.6405361
TGGCGAG10400.010.10316818
TCTATAC2250.010.0968333
GGCGTGC856.816746E-410.0225928
TACACCG856.816746E-410.0225925
GTCCTAC8850.09.9666041
GTGCGCC1252.6913876E-69.84441311
TAGGACA3500.09.7362334
AGGACGT11000.09.7239395
Produced by FastQC (version 0.11.5)