FastQCFastQC Report
Thu 26 May 2016
SRR1512009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895929
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35230.18581919470613087No Hit
GGTATCAACGCAGAGTACTTTTTTT21370.11271519133891617No Hit
TATCAACGCAGAGTACTTTTTTTTT21040.11097461983017297No Hit
GTCCTACAGTGGACATTTCTAAATT19160.1010586366894541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.012.5850271
GGCGAGG4600.010.94106319
GTCCTAA5450.010.863271
CGTGCGC1106.0900547E-610.35737810
GTTCTAG3050.010.0187961
GTATCAA18550.09.9867281
GGCGTGC1252.590028E-69.8740348
GTCCTAC10450.09.8689931
AGGACGT10450.09.8241715
GGACGTG10600.09.7691536
TTAGGAC6400.09.6543243
TAGGACC3350.09.6476734
CAGGACT3750.09.632544
TAGGACG11200.09.590674
GACGTGG5150.09.5864417
GTATGAT1002.6163034E-49.5491651
GCCTAAG1701.529952E-89.5491651
TGGCGAG8950.09.54780818
TAGAGTG2800.09.5057965
TTTAGGA7650.09.1951482
Produced by FastQC (version 0.11.5)