FastQCFastQC Report
Thu 26 May 2016
SRR1512008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697972
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25070.14764672208964574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCC456.9486874E-414.7250079
CGTGCGC550.003151956512.04773310
GGTATCA7600.011.8613091
TAGGACC2900.011.7509924
TAGACAG2250.010.9385775
GTAATAC1201.5950682E-610.2549173
GGCGAGG3550.09.86605219
GTATCAA14600.09.8655851
ACTAGAC1252.6896978E-69.844723
TTAGCAC1752.73576E-99.7365363
TATACTG2650.09.6446825
AGGACGT7350.09.5304725
GTCCTAA4450.09.4681871
ATTAGGG1401.1111097E-69.4660773
TGGACCG1002.8559336E-49.4660775
TAGGACT1901.1204975E-99.4660774
CCTATAC1502.7883834E-79.4660773
GTCCTAG2302.1827873E-119.3724521
GTAGGAC7500.09.3398633
GTCTTAA2001.5188562E-99.3085491
Produced by FastQC (version 0.11.5)