FastQCFastQC Report
Thu 26 May 2016
SRR1512008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697972
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30390.17897821636634761No Hit
GGTATCAACGCAGAGTACTTTTTTT19010.11195708763159816No Hit
TATCAACGCAGAGTACTTTTTTTTT18070.10642107172556439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA751.4843707E-512.66201219
TCCAACG1052.7374153E-711.75619818
GGTATCA8300.011.61654951
GTCCTAC8500.011.0062921
GGCGAGG3650.010.92749119
GTAGGAC7700.010.7380463
GGACGTG6950.010.5259636
GTATTAG1002.2734568E-510.5009011
TATACTG3000.010.4541655
CTGGTCG1106.082353E-610.3583049
TCTAATC1201.5157639E-610.295773
TGGCGAG8600.010.26827218
TAGACAG2500.010.2640915
TTAGGGC750.002639045710.1373733
GCCCTAA856.305472E-410.107821
GTGTACG856.305472E-410.107821
TAGGACG7550.010.0702384
AGGACGT7200.010.0317755
TTGCACC951.6374257E-410.0039864
TTAGACT1151.0214666E-59.9169954
Produced by FastQC (version 0.11.5)