FastQCFastQC Report
Thu 26 May 2016
SRR1512007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650461
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28280.1713460663414646No Hit
GTCCTACAGTGGACATTTCTAAATT17790.10778806648566673No Hit
GGTATCAACGCAGAGTACTTTTTTT17590.10657628383827307No Hit
TATCAACGCAGAGTACTTTTTTTTT17230.10439507507296446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG250.00615874618.9318374
CGATTAT450.00937984813.0645051
GGTATCA7250.012.5689541
GCGCCAC1104.0095983E-812.04753313
ACCGTGC1104.0095983E-812.0475338
GCGTGCG951.0860113E-611.9569499
TAGGACC4950.011.4738414
CGGTTGA600.00603519811.04357114
GTATAAG1707.8216544E-1110.951131
TAGCACT1658.54925E-1110.9001484
GTGCGTT700.001536872410.818192511
TTAGGAC6050.010.7958413
GTATCAA15200.010.5719361
TGCGCCA1451.5259502E-810.44515112
GTGCGCC1106.3095904E-610.32645711
AGGACCT8200.010.2739845
GGACGTG9900.010.2308416
AGGACGT10350.010.15185455
TGTAGGA11700.010.1333662
TTAGTAC750.002727829910.096983
Produced by FastQC (version 0.11.5)