##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512007_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1650461 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.11684553588361 31.0 28.0 34.0 22.0 34.0 2 26.24683891349144 31.0 25.0 34.0 10.0 34.0 3 28.17811932544907 31.0 27.0 34.0 16.0 34.0 4 32.63100430728142 35.0 33.0 37.0 25.0 37.0 5 33.06466375152154 35.0 35.0 37.0 27.0 37.0 6 33.33540083649356 37.0 35.0 37.0 28.0 37.0 7 33.39612084138916 37.0 35.0 37.0 28.0 37.0 8 33.426869220175455 37.0 35.0 37.0 28.0 37.0 9 35.01359196006449 39.0 35.0 39.0 28.0 39.0 10 34.92028166675856 39.0 35.0 39.0 28.0 39.0 11 34.92452835904635 39.0 35.0 39.0 27.0 39.0 12 34.81124182879814 39.0 35.0 39.0 27.0 39.0 13 34.80911393846931 39.0 35.0 39.0 27.0 39.0 14 35.96622216459522 40.0 36.0 41.0 27.0 41.0 15 35.900609587260774 40.0 36.0 41.0 27.0 41.0 16 35.840146480286414 40.0 36.0 41.0 27.0 41.0 17 35.802411568646576 40.0 36.0 41.0 27.0 41.0 18 35.743783706491705 40.0 36.0 41.0 27.0 41.0 19 35.76348850412097 40.0 36.0 41.0 27.0 41.0 20 35.659700532154346 40.0 36.0 41.0 27.0 41.0 21 35.60158828351594 40.0 36.0 41.0 27.0 41.0 22 35.48790247088541 40.0 36.0 41.0 26.0 41.0 23 35.385523802137705 40.0 36.0 41.0 25.0 41.0 24 35.28509973880025 40.0 36.0 41.0 25.0 41.0 25 35.17492930762981 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1918040911063272 1101 2 4.033889504785591 1101 3 2.2541225834049854 1101 4 1.3321055992333868 1101 5 1.0555418476497422 1101 6 0.695073544003364 1101 7 0.24001042679649487 1101 8 0.245772828619657 1101 9 0.26615146518505384 1101 10 0.4573013335538292 1101 11 0.32002992777442074 1101 12 0.17555250716522863 1101 13 0.18927872305602023 1101 14 0.0069808385018390595 1101 15 0.19348213760401478 1101 16 0.1729537480790384 1101 17 0.273687356482192 1101 18 0.14009329549089244 1101 19 0.18327798960747543 1101 20 0.2234628307481188 1101 21 0.07022935548881293 1101 22 0.1809559660738671 1101 23 0.09034453634195927 1101 24 0.10992475706326843 1101 25 -0.061766681298081494 1102 1 1.9563264463888963 1102 2 4.605684618535033 1102 3 2.8905215471355525 1102 4 2.060438303484837 1102 5 1.6120039483257216 1102 6 1.4031595337779237 1102 7 1.2810743186401652 1102 8 1.2874612272059451 1102 9 1.443211994300107 1102 10 1.1768343260391632 1102 11 1.2809090411121034 1102 12 1.3480338920603998 1102 13 1.4227490246721928 1102 14 1.550220209877473 1102 15 1.3481796635965324 1102 16 1.4159494931979353 1102 17 1.3733416473925288 1102 18 1.4608143948972625 1102 19 1.5987894331788297 1102 20 1.61614803817357 1102 21 1.526714617815962 1102 22 1.428361003304076 1102 23 1.535348482401048 1102 24 1.5414760729881962 1102 25 1.490074241050344 1103 1 1.4751586504542615 1103 2 4.234089029322863 1103 3 2.598994378276778 1103 4 1.8560740149236565 1103 5 1.4010334421479627 1103 6 1.2274686874912248 1103 7 1.091483512209976 1103 8 1.0097310558229182 1103 9 1.0782757647265697 1103 10 0.8738915865679218 1103 11 0.9954799192840156 1103 12 1.0104990176084314 1103 13 1.001003973522991 1103 14 1.0961980230832609 1103 15 1.1389694868993132 1103 16 1.049787675740781 1103 17 0.990642696947468 1103 18 1.174551607598211 1103 19 1.0572124436743806 1103 20 1.1522580421592252 1103 21 1.237210167171611 1103 22 1.2134912012581083 1103 23 1.3898223763827176 1103 24 1.3512475622933593 1103 25 1.5464180699330328 1104 1 1.7002633724649243 1104 2 4.186377046586131 1104 3 2.480236444791121 1104 4 1.9647469014430783 1104 5 1.3771568237168879 1104 6 1.2429802386463393 1104 7 0.9611934639743822 1104 8 1.1264675371633643 1104 9 1.1756672346427948 1104 10 1.222219207791582 1104 11 1.011594415349009 1104 12 1.1407351181415564 1104 13 1.0928293099027826 1104 14 1.2329332350659996 1104 15 1.1909052573994359 1104 16 1.174330706966984 1104 17 1.1158870483056802 1104 18 1.228410455021411 1104 19 1.3866171149107345 1104 20 1.2247447139033198 1104 21 1.100154442368364 1104 22 1.0986048087727056 1104 23 1.3626422545243742 1104 24 1.3704339040044573 1104 25 1.3623104283996454 1105 1 1.483331436530861 1105 2 4.232722050868773 1105 3 2.5887868302289405 1105 4 1.6065626370093753 1105 5 1.2396345869197063 1105 6 1.0552515066079806 1105 7 0.8284873281157914 1105 8 0.6743245687830424 1105 9 0.6455427385271193 1105 10 0.7408700406607167 1105 11 0.9650678479917261 1105 12 0.7846858108514851 1105 13 0.7950823391984585 1105 14 0.8949369127477453 1105 15 0.9213383733063125 1105 16 1.1403840273625434 1105 17 1.1856098894645655 1105 18 0.9531720645762007 1105 19 1.0647697986001532 1105 20 0.8740936324612392 1105 21 0.9854511276630262 1105 22 0.9798786553043684 1105 23 0.8274590742281234 1105 24 0.9065526020811561 1105 25 0.9507784403551085 1106 1 0.6262101851264852 1106 2 2.271666394486438 1106 3 1.2606496770010445 1106 4 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-0.2650751721578999 2314 7 -0.3436061916877762 2314 8 -0.29451995632283 2314 9 -0.4264475821020426 2314 10 -0.5531942275147941 2314 11 -0.3920190884162338 2314 12 -0.6506901544166581 2314 13 -0.6774631218171692 2314 14 -0.6089063668508459 2314 15 -0.6395313466324808 2314 16 -0.7978566046434352 2314 17 -0.778750809442947 2314 18 -0.667944621662727 2314 19 -0.7066440300892154 2314 20 -0.7045868415432466 2314 21 -0.6365018277478143 2314 22 -0.5932326218743853 2314 23 -0.6511893724463746 2314 24 -0.7554167228538944 2314 25 -0.8510257659009 2315 1 -3.109669772488914 2315 2 -11.009572363928692 2315 3 -4.918654366421148 2315 4 -2.5542874815034153 2315 5 -1.739258506340711 2315 6 -1.389359429746726 2315 7 -1.246379035078796 2315 8 -1.3932858726038404 2315 9 -1.4953418744648417 2315 10 -1.5881627525355952 2315 11 -1.632726164249938 2315 12 -1.4046677828042746 2315 13 -1.4770444807549694 2315 14 -1.6598453870585104 2315 15 -1.9242226488338119 2315 16 -1.911318021840323 2315 17 -1.9821246995904218 2315 18 -1.9306195873270866 2315 19 -1.7817802576134056 2315 20 -1.8528806715860497 2315 21 -1.849497387568725 2315 22 -1.8199109157505617 2315 23 -1.9845757664580503 2315 24 -1.9789159900013402 2315 25 -1.861534087199452 2316 1 -0.814991949681616 2316 2 -0.36543979481341893 2316 3 -0.3031885058982553 2316 4 -0.5580386143508989 2316 5 -1.1907533775093206 2316 6 -1.2615973903628799 2316 7 -1.4206632631605487 2316 8 -1.494922828075321 2316 9 -1.4754722536926934 2316 10 -1.6769375319746374 2316 11 -1.8647330849181927 2316 12 -1.5100339332483657 2316 13 -1.7808699098719885 2316 14 -1.847892462812915 2316 15 -1.733632485874402 2316 16 -1.7748914969020788 2316 17 -2.164352144651893 2316 18 -2.0721086425213144 2316 19 -1.8706932456640857 2316 20 -1.9456830010958583 2316 21 -1.8913334024416315 2316 22 -2.0652989650688554 2316 23 -2.170559219450304 2316 24 -2.0402737884527227 2316 25 -2.03480283542919 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66207.0 3 2.0 4 550.0 5 2637.0 6 3115.0 7 2832.0 8 2660.0 9 2590.0 10 2409.0 11 2424.0 12 2328.0 13 2451.0 14 2387.0 15 2456.0 16 2654.0 17 2872.0 18 3287.0 19 4000.0 20 5229.0 21 6665.0 22 8176.0 23 9584.0 24 11070.0 25 12645.0 26 14238.0 27 16027.0 28 18616.0 29 22359.0 30 28099.0 31 35197.0 32 45022.0 33 59261.0 34 81211.0 35 116393.0 36 184307.0 37 257934.0 38 610567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.072450666813694 18.825043427260628 12.773097940514802 25.329407965410876 2 17.37430234169844 21.4402414388491 38.39433381354343 22.791122405909032 3 18.58117216947265 24.37609855670628 29.70182270286908 27.340906570951994 4 12.460458017487236 16.840264629094538 38.28542449654975 32.41385285686847 5 13.438003079139706 37.40857857289569 35.76909724010443 13.384321107860167 6 31.633806110906114 37.24339713982595 18.11605135298726 13.006745396280673 7 27.962066356005987 31.592082454538463 22.40670939816209 18.03914179129346 8 25.20921440083371 36.5765907686342 20.6084970250718 17.605697805460295 9 26.22455180704058 15.63811565374765 20.333470466736266 37.80386207247551 10 14.938441404214583 28.864377203931024 34.35654302436896 21.84063836748543 11 34.19117446580076 22.80671885006674 24.40645371202349 18.595652972109004 12 23.115844603416864 24.696736245206644 31.523010843637024 20.664408307739475 13 28.802619389370605 21.896912438403575 25.81872579842844 23.48174237379738 14 21.163056867141968 21.796576835199378 27.497590067259996 29.54277623039866 15 22.99587812132489 30.341159227633973 23.502403267935442 23.16055938310569 16 22.547397363524492 28.261619026441704 26.919812100982693 22.271171509051108 17 21.998399235122793 28.35813751430661 27.48329103202075 22.16017221854985 18 21.877300501982784 27.61692987691273 28.893365768833444 21.61240385227104 19 23.360132036477257 27.201077522352957 27.489180243242313 21.949610197927477 20 23.635300568631074 27.107009885152884 27.77143272612704 21.486256820089007 21 23.294393514338864 26.722269011942483 27.149132639768297 22.834204833950352 22 23.367885819262305 27.53345176668468 27.21444515267481 21.884217261378204 23 22.628919385622105 27.34951073949529 27.555575752067618 22.46599412281499 24 23.07291486792462 26.756496223171855 28.070009615506958 22.100579293396564 25 22.905487937878984 27.10868996204685 27.730457522042396 22.25536457803177 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 168.0 1 168.0 2 303.0 3 438.0 4 438.0 5 438.0 6 986.5 7 1535.0 8 1535.0 9 1535.0 10 2420.5 11 3306.0 12 3306.0 13 3306.0 14 5377.0 15 7448.0 16 7448.0 17 7448.0 18 13191.5 19 18935.0 20 18935.0 21 18935.0 22 29694.0 23 40453.0 24 40453.0 25 40453.0 26 57259.5 27 74066.0 28 74066.0 29 74066.0 30 94480.0 31 114894.0 32 114894.0 33 114894.0 34 133148.5 35 151403.0 36 151403.0 37 151403.0 38 166190.0 39 180977.0 40 180977.0 41 180977.0 42 193105.0 43 205233.0 44 205233.0 45 205233.0 46 210827.0 47 216421.0 48 216421.0 49 216421.0 50 211080.0 51 205739.0 52 205739.0 53 205739.0 54 185559.5 55 165380.0 56 165380.0 57 165380.0 58 142498.5 59 119617.0 60 119617.0 61 119617.0 62 97332.0 63 75047.0 64 75047.0 65 75047.0 66 57710.0 67 40373.0 68 40373.0 69 40373.0 70 29461.5 71 18550.0 72 18550.0 73 18550.0 74 12739.5 75 6929.0 76 6929.0 77 6929.0 78 4676.5 79 2424.0 80 2424.0 81 2424.0 82 1600.0 83 776.0 84 776.0 85 776.0 86 511.0 87 246.0 88 246.0 89 246.0 90 159.5 91 73.0 92 73.0 93 73.0 94 45.5 95 18.0 96 18.0 97 18.0 98 15.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.3942031953496627 3 0.0 4 0.0 5 0.0 6 1.2117826473936675E-4 7 0.0 8 6.0589132369683376E-5 9 0.0 10 6.0589132369683376E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.6353479421810024E-4 19 4.84713058957467E-4 20 3.6353479421810024E-4 21 0.0030900457508538526 22 0.0015147283092420845 23 6.664804560665171E-4 24 1.2117826473936675E-4 25 3.029456618484169E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1650461.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.17048728375458 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22328305409779 62.55934740530367 2 11.666156102813584 17.539012779536893 3 2.9433912686391337 6.637684677910567 4 1.0076367290517836 3.029781757113246 5 0.4285391531032314 1.6106748479468709 6 0.2168462734168806 0.9780264023047917 7 0.13029832227258928 0.685621186424036 8 0.08359926173586953 0.5027357792997961 9 0.05477391962589008 0.370564400584743 >10 0.2222547443836435 2.817914236363873 >50 0.013450280044292849 0.6943073781086649 >100 0.008061255319061432 1.1989219363709571 >500 0.001058345783440194 0.5398205572195002 >1k 6.512897128862732E-4 0.8355866555124201 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2828 0.1713460663414646 No Hit GTCCTACAGTGGACATTTCTAAATT 1779 0.10778806648566673 No Hit GGTATCAACGCAGAGTACTTTTTTT 1759 0.10657628383827307 No Hit TATCAACGCAGAGTACTTTTTTTTT 1723 0.10439507507296446 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2117826473936677E-4 2 0.0 0.0 0.0 0.0 1.2117826473936677E-4 3 0.0 0.0 0.0 0.0 1.2117826473936677E-4 4 0.0 0.0 0.0 6.058913236968338E-5 1.2117826473936677E-4 5 0.0 0.0 0.0 6.058913236968338E-5 1.8176739710905015E-4 6 0.0 0.0 0.0 6.058913236968338E-5 1.8176739710905015E-4 7 0.0 0.0 0.0 6.058913236968338E-5 1.8176739710905015E-4 8 0.0 0.0 0.0 6.058913236968338E-5 1.8176739710905015E-4 9 0.0 0.0 0.0 1.2117826473936677E-4 1.8176739710905015E-4 10 0.0 0.0 0.0 1.2117826473936677E-4 1.8176739710905015E-4 11 0.0 0.0 0.0 1.2117826473936677E-4 1.8176739710905015E-4 12 0.0 6.058913236968338E-5 0.0 1.2117826473936677E-4 1.8176739710905015E-4 13 0.0 6.058913236968338E-5 0.0 1.2117826473936677E-4 1.8176739710905015E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCCG 25 0.006158746 18.931837 4 CGATTAT 45 0.009379848 13.064505 1 GGTATCA 725 0.0 12.568954 1 GCGCCAC 110 4.0095983E-8 12.047533 13 ACCGTGC 110 4.0095983E-8 12.047533 8 GCGTGCG 95 1.0860113E-6 11.956949 9 TAGGACC 495 0.0 11.473841 4 CGGTTGA 60 0.006035198 11.043571 14 GTATAAG 170 7.8216544E-11 10.95113 1 TAGCACT 165 8.54925E-11 10.900148 4 GTGCGTT 70 0.0015368724 10.8181925 11 TTAGGAC 605 0.0 10.795841 3 GTATCAA 1520 0.0 10.571936 1 TGCGCCA 145 1.5259502E-8 10.445151 12 GTGCGCC 110 6.3095904E-6 10.326457 11 AGGACCT 820 0.0 10.273984 5 GGACGTG 990 0.0 10.230841 6 AGGACGT 1035 0.0 10.1518545 5 TGTAGGA 1170 0.0 10.133366 2 TTAGTAC 75 0.0027278299 10.09698 3 >>END_MODULE