FastQCFastQC Report
Thu 26 May 2016
SRR1512007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650461
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32440.19655114540725288No Hit
GGTATCAACGCAGAGTACTTTTTTT19820.12008766035671245No Hit
TATCAACGCAGAGTACTTTTTTTTT19560.1185123429151007No Hit
GTCCTACAGTGGACATTTCTAAATT19470.11796704072377354No Hit
GTCCTAAAGTGTGTATTTCTCATTT18530.1122716622810233No Hit
CTGTAGGACGTGGAATATGGCAAGA17940.10869690347121198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC350.002102746916.3629531
TAGGACC4150.013.511244
GGTATCA8150.011.8288431
GGCGAGG4500.011.39645519
TGGCGAG9500.010.894629518
GGACCGT700.001491963310.8567276
GTATCAA16550.010.7273741
CCTAGAC1605.4023985E-1010.69162753
ACCGTGC803.7881514E-410.6822358
GTCCTAC11350.010.5121761
AGGACCT8300.010.1906815
GGACCTG7300.010.1502966
TGTAGGA12100.010.132012
GGCGAGA5550.09.92484119
GTATTAG1451.5087426E-79.8741961
GTATAAC1553.7747668E-89.8529611
CCAGGAC4550.09.8169773
CCTACAG12000.09.7412613
TATGGCG10750.09.71614116
TAAGACA2251.8189894E-129.7148624
Produced by FastQC (version 0.11.5)