FastQCFastQC Report
Thu 26 May 2016
SRR1512006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038541
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26660.13077980771542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.012.3041621
TAGGACC5300.011.7871384
CGTCTAA600.004651581411.436561
GTAGGAC13450.011.3303643
GACGTGG6250.010.9041737
GGACGTG12500.010.9041736
AGGACGT12700.010.8815165
TAGGACG13650.010.6789454
GTATCAA16350.010.5522131
TGTAGGA13850.010.1920462
CTGTAGG12950.010.1434031
GGGCCGT750.002729473910.0964576
ACGAAAT750.002729473910.09645712
TGGCGAG11650.010.07553218
ACCGTGC1054.2736887E-59.91616258
GACGTGA7000.09.7358687
GTCCTAA6950.09.7322441
TATGGCG12800.09.61403316
ACGTGGA7300.09.5950918
GTATTAG1552.703364E-79.4865481
Produced by FastQC (version 0.11.5)