FastQCFastQC Report
Thu 26 May 2016
SRR1512006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038541
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32560.1597220757394627No Hit
GTCCTACAGTGGACATTTCTAAATT24110.11827086136604562No Hit
CTGTAGGACGTGGAATATGGCAAGA21210.10404500081185515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1501.8189894E-1212.66043618
GGTATCA11400.012.1438161
GTCCTAC12650.011.3966831
GTACTAG1104.6597415E-711.283471
CTTATAC2050.011.1271253
GTAGGAC13200.010.8724793
AGGACGT11850.010.7476145
TAGGACC4700.010.717754
TCCTATA2350.010.5155282
GACGTGG6250.010.4823277
GTCCTAA6850.010.4535251
GGACGTG12200.010.2786756
TAGGACG12600.010.2587384
CCAACGA1303.8632425E-710.22674119
TCCTACA14900.010.2060882
GCCTTAG1909.094947E-1110.0500551
CCTACAC3050.09.9718493
TGTAGGA14300.09.969672
CTGTAGG14300.09.747851
TCTATAC2251.8189894E-129.7156293
Produced by FastQC (version 0.11.5)