FastQCFastQC Report
Thu 26 May 2016
SRR1512005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980849
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30300.15296471361522257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2300.011.921891
GGTATCA8000.011.873991
GGTATAC959.712641E-611.3392411
GTATCAA19100.011.2286021
GTCCTAT1853.274181E-1110.5870071
GTTTAGT1302.53076E-710.5462881
TAGACAG3050.010.2426385
CTTACAC2500.09.8453473
GTCTTAC1002.030275E-49.7929811
GTGGTAT3900.09.7929811
TGGTATC3450.09.6510532
GTTCTAC2750.09.6149261
TCTGGAC3850.09.5896243
TAGACTG2700.09.4666815
GTAGGAC2401.8189894E-129.4666813
CCCGTTT800.0046491559.46668112
TAATGCT1752.8263457E-89.1962034
TTAGACT1651.12469934E-79.1798114
TATACTG2700.09.1160635
GTGTTAT1303.0565738E-59.0396751
Produced by FastQC (version 0.11.5)