##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512005_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1980849 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.450982886630936 31.0 28.0 33.0 16.0 34.0 2 25.679631814439162 31.0 22.0 34.0 10.0 34.0 3 27.551422647561726 31.0 26.0 34.0 16.0 34.0 4 31.90140793164951 35.0 32.0 37.0 19.0 37.0 5 32.296522854594166 35.0 33.0 37.0 20.0 37.0 6 32.551162658031984 36.0 33.0 37.0 23.0 37.0 7 32.59120760845476 37.0 35.0 37.0 23.0 37.0 8 32.62848606834746 37.0 35.0 37.0 23.0 37.0 9 34.14008134895694 39.0 35.0 39.0 25.0 39.0 10 34.03911706546032 39.0 35.0 39.0 23.0 39.0 11 34.047953680467316 39.0 35.0 39.0 23.0 39.0 12 33.90443946004971 39.0 35.0 39.0 22.0 39.0 13 33.90240094020292 39.0 35.0 39.0 22.0 39.0 14 34.997143144177066 40.0 36.0 41.0 22.0 41.0 15 34.8995668019117 40.0 35.0 41.0 21.0 41.0 16 34.83363093299893 40.0 35.0 41.0 19.0 41.0 17 34.7769062659496 40.0 35.0 41.0 19.0 41.0 18 34.704199058080654 40.0 35.0 41.0 18.0 41.0 19 34.71380100148976 40.0 35.0 41.0 18.0 41.0 20 34.59355660123513 40.0 35.0 41.0 18.0 41.0 21 34.52283742980914 40.0 35.0 41.0 18.0 41.0 22 34.39854880407341 39.0 34.0 41.0 16.0 41.0 23 34.27998297699623 39.0 34.0 41.0 10.0 41.0 24 34.15010886746037 39.0 34.0 41.0 2.0 41.0 25 34.023541420875596 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7835463415858221 1101 2 4.5390855910263355 1101 3 2.8069602701481777 1101 4 1.937506492551286 1101 5 1.5055794136275367 1101 6 1.2491522942768185 1101 7 0.7318387152682391 1101 8 0.7765788319956712 1101 9 0.9300558684356446 1101 10 1.1441857443631633 1101 11 1.0562655272785193 1101 12 0.8336405322207128 1101 13 0.9404211842180032 1101 14 0.7225128881811571 1101 15 1.0246600117734417 1101 16 1.0269289613298511 1101 17 1.097173910018661 1101 18 1.3004878911803104 1101 19 1.151487679333755 1101 20 1.217124136145415 1101 21 1.2147971403271 1101 22 1.1994674381389743 1101 23 1.3159132427303533 1101 24 1.2201200825586085 1101 25 1.0571137152146122 1102 1 2.2531999384531893 1102 2 4.693344517512035 1102 3 3.087705860144382 1102 4 2.4202844711244396 1102 5 1.8993179350608855 1102 6 1.7209207472304655 1102 7 1.5651584737029154 1102 8 1.4551741580449402 1102 9 1.6540334947401831 1102 10 1.3364541042721925 1102 11 1.5912101445201117 1102 12 1.659841104742064 1102 13 1.5812293658094276 1102 14 1.898936230445642 1102 15 1.8880388379858957 1102 16 1.9293002698765562 1102 17 1.8789345064997107 1102 18 1.9078899440443635 1102 19 1.9894053690670006 1102 20 1.9489457913893702 1102 21 1.9791422730754675 1102 22 2.0287025661304767 1102 23 2.042937019730971 1102 24 1.9420182040754455 1102 25 1.9780475664303268 1103 1 1.4237849782195546 1103 2 4.128308346847092 1103 3 2.374198778884267 1103 4 1.7566280960482104 1103 5 1.3836522111565088 1103 6 1.3912788359810762 1103 7 1.0457832937096754 1103 8 0.9937133814507462 1103 9 1.0696623737852775 1103 10 1.0226671352557233 1103 11 0.9630230503199044 1103 12 0.7629170955996543 1103 13 1.0135452483298124 1103 14 1.1233616531765946 1103 15 1.1998662042831683 1103 16 1.0925415853933984 1103 17 1.0621339165380448 1103 18 1.1722082503350109 1103 19 1.2959271311169118 1103 20 1.2991059610976805 1103 21 1.4889219752202933 1103 22 1.2391796571178517 1103 23 1.5378716839183753 1103 24 1.385726028780475 1103 25 1.3479422089991218 1104 1 1.716059137478748 1104 2 4.185034544622624 1104 3 2.478111076397287 1104 4 1.962844407000496 1104 5 1.5865179302884655 1104 6 1.2638635611891331 1104 7 1.0091780102468917 1104 8 1.1712708732655344 1104 9 1.0803283791961462 1104 10 1.035944160559609 1104 11 1.0700265740030233 1104 12 1.2929801789688895 1104 13 1.1857461340797855 1104 14 1.3251687480751215 1104 15 1.2580292226482115 1104 16 1.4155120491804212 1104 17 1.1453419983282984 1104 18 1.091682812424871 1104 19 1.2538404422178715 1104 20 1.2429869393945268 1104 21 1.0382768709911119 1104 22 1.2465824302684112 1104 23 1.374761075863283 1104 24 1.483331074581848 1104 25 1.3840303889793546 1105 1 1.6829982316267689 1105 2 4.394349980075518 1105 3 2.623687609929842 1105 4 1.8765591493005047 1105 5 1.4285827410276113 1105 6 1.1270655476817808 1105 7 1.0391991584400628 1105 8 0.854340461890942 1105 9 0.9147206086647941 1105 10 0.9199134523968056 1105 11 1.0171615997864052 1105 12 1.0419874310520996 1105 13 0.9550068077265976 1105 14 0.8992393634188147 1105 15 1.0223771778419533 1105 16 1.0845478101402577 1105 17 1.2566223637707594 1105 18 1.0921623323593082 1105 19 1.0608208149528053 1105 20 1.0948163289299728 1105 21 1.0846135013300326 1105 22 1.2329444426362457 1105 23 1.0945399090978967 1105 24 1.2629163790271605 1105 25 1.3770073106397476 1106 1 0.7461125745197563 1106 2 2.3222551914788667 1106 3 1.4372866296433493 1106 4 0.8604801040098096 1106 5 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0.9330064852211208 1107 18 0.8604841826165313 1107 19 0.83935014008172 1107 20 0.8702011641740484 1107 21 1.0209949195084604 1107 22 1.0233211942977434 1107 23 1.0983372316594497 1107 24 1.042046070371974 1107 25 1.1838325476070182 1108 1 1.4894994427294854 1108 2 3.5433949070092297 1108 3 2.2058510726881906 1108 4 1.5870192317062362 1108 5 1.056233553081256 1108 6 0.8482223215617779 1108 7 0.7338861162007859 1108 8 0.6233411606415871 1108 9 0.7348655115911527 1108 10 0.7781634431256705 1108 11 0.6704873503090027 1108 12 0.7495798056917451 1108 13 0.8890751467104465 1108 14 0.7574915499422943 1108 15 0.7192953069078314 1108 16 0.8156742235000607 1108 17 0.8120481234668944 1108 18 0.7331813860011067 1108 19 0.8432849063915455 1108 20 0.8846946428720059 1108 21 0.8645856098588922 1108 22 0.9991225693989847 1108 23 1.0367336640628295 1108 24 0.8262335805893954 1108 25 0.8073837869456639 1109 1 0.9482212620773218 1109 2 3.1839754572205337 1109 3 1.904498976919193 1109 4 1.1240137887910677 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-1.8896125590288833 2315 24 -1.8715612801006571 2315 25 -1.6792122664784728 2316 1 -1.2748326567574928 2316 2 -1.0175798316101599 2316 3 -1.0000064505276036 2316 4 -1.361186820634824 2316 5 -1.681968602902895 2316 6 -1.809833396341979 2316 7 -1.8395634584469214 2316 8 -2.0104922051613876 2316 9 -2.088816220988882 2316 10 -2.3327431092478577 2316 11 -2.397396927890881 2316 12 -2.265253949746917 2316 13 -2.4818580307686133 2316 14 -2.5978042484861774 2316 15 -2.4530990044110865 2316 16 -2.5239030217756095 2316 17 -2.626766085247951 2316 18 -2.608085904082664 2316 19 -2.5524583236883274 2316 20 -2.428785128541726 2316 21 -2.624681550346317 2316 22 -2.7025788397834347 2316 23 -2.6282250994932426 2316 24 -2.642975458958624 2316 25 -2.6650348766039187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108899.0 3 2.0 4 915.0 5 4153.0 6 4955.0 7 4360.0 8 3936.0 9 3961.0 10 3745.0 11 3655.0 12 3578.0 13 3428.0 14 3625.0 15 3643.0 16 3857.0 17 4214.0 18 4802.0 19 5902.0 20 7527.0 21 9739.0 22 11666.0 23 13693.0 24 15645.0 25 17311.0 26 19697.0 27 21836.0 28 24952.0 29 30033.0 30 36572.0 31 45353.0 32 57382.0 33 75308.0 34 101651.0 35 142579.0 36 216228.0 37 304998.0 38 657049.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.973392722009606 17.273552905849968 12.597477142376828 25.155577229763605 2 18.41032065881257 20.333772186591144 36.83667399713501 24.419233157461278 3 19.60381634339619 22.498181335376902 28.43144530451337 29.46655701671354 4 13.33887641107424 15.370883898772696 35.52663529627952 35.763604393873536 5 14.935716957728731 36.17241899811646 34.10815261536846 14.783711428786345 6 34.77436672292206 34.72034942628078 16.617840751961154 13.887443098836002 7 30.402418356977236 30.48798772647486 20.158427017909997 18.951166898637908 8 27.291429079147377 34.95521364828919 18.743276241651937 19.010081030911493 9 26.655540124461787 15.736333259122729 18.783259097488 38.82486751892749 10 15.946301785901795 28.206404529777146 32.16521409012706 23.682079594194004 11 36.20553611103118 22.24833896980537 21.995164699580837 19.550960219582613 12 23.92161138986364 23.774755168112257 29.533750427215804 22.769883014808297 13 29.408501102305124 20.48429738965464 25.071370912169478 25.035830595870763 14 22.697994647749525 19.998546077969596 25.94852005377492 31.354939220505955 15 24.59127374171378 27.996631747296234 22.491416559263225 24.92067795172676 16 24.50676452369666 27.22559872054861 24.3511746730821 23.91646208267263 17 23.735176179506865 27.017304196331978 25.584888095962892 23.662631528198265 18 23.6275253200918 26.866810915380256 26.255150597878274 23.250513166649668 19 24.702284341991476 26.224623681047316 25.9765462308029 23.09654574615831 20 24.861106693786517 25.799699823862337 26.106893197168674 23.232300285182475 21 24.99079910237608 25.47076032986922 25.728234008234107 23.810206559520594 22 24.87656602127608 25.35147131283269 25.957686125314012 23.814276540577218 23 24.73680224956205 25.40621860533211 25.746429527016453 24.110549618089387 24 24.29947966755669 25.706149231970738 25.939887391719406 24.054483708753168 25 24.500981654743267 25.484554777041446 25.984544932728 24.029918635487284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 89.0 1 89.0 2 203.5 3 318.0 4 318.0 5 318.0 6 781.5 7 1245.0 8 1245.0 9 1245.0 10 1762.5 11 2280.0 12 2280.0 13 2280.0 14 3784.0 15 5288.0 16 5288.0 17 5288.0 18 9770.5 19 14253.0 20 14253.0 21 14253.0 22 22109.5 23 29966.0 24 29966.0 25 29966.0 26 42945.0 27 55924.0 28 55924.0 29 55924.0 30 73981.0 31 92038.0 32 92038.0 33 92038.0 34 112332.0 35 132626.0 36 132626.0 37 132626.0 38 156751.0 39 180876.0 40 180876.0 41 180876.0 42 203393.0 43 225910.0 44 225910.0 45 225910.0 46 244439.0 47 262968.0 48 262968.0 49 262968.0 50 270664.0 51 278360.0 52 278360.0 53 278360.0 54 263068.0 55 247776.0 56 247776.0 57 247776.0 58 219299.5 59 190823.0 60 190823.0 61 190823.0 62 159737.5 63 128652.0 64 128652.0 65 128652.0 66 101395.0 67 74138.0 68 74138.0 69 74138.0 70 54606.5 71 35075.0 72 35075.0 73 35075.0 74 24737.0 75 14399.0 76 14399.0 77 14399.0 78 9892.0 79 5385.0 80 5385.0 81 5385.0 82 3570.0 83 1755.0 84 1755.0 85 1755.0 86 1131.0 87 507.0 88 507.0 89 507.0 90 324.5 91 142.0 92 142.0 93 142.0 94 92.5 95 43.0 96 43.0 97 43.0 98 28.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.3324094870431824 3 0.0 4 0.0 5 0.0 6 1.0096680766681356E-4 7 0.0 8 0.0 9 0.0 10 5.048340383340678E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.553174421674747E-4 19 8.077344613345085E-4 20 6.058008460008814E-4 21 0.003230937845338034 22 0.0016659523265024241 23 9.59184672834729E-4 24 0.0 25 5.048340383340678E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1980849.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.80975109868949 #Duplication Level Percentage of deduplicated Percentage of total 1 83.98614334792256 61.15010192901098 2 10.018853673431096 14.58940484513418 3 2.799365864243424 6.114633954291944 4 1.2024295743170998 3.501943920789248 5 0.5922581610530774 2.1561084646221063 6 0.36842334429882057 1.6094887198406342 7 0.2417140262771044 1.2319396663208644 8 0.16756319982287876 0.9760187897923017 9 0.11443472921296172 0.7498767735937507 >10 0.48863705316795714 5.7813951940428545 >50 0.014496912601309956 0.7216273174830921 >100 0.004629246954584708 0.5750485224440434 >500 7.005777980801473E-4 0.3636934220878361 >1k 3.502888990400697E-4 0.478718480546187 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3030 0.15296471361522257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.048340383340679E-5 0.0 0.0 0.0 3 0.0 5.048340383340679E-5 0.0 0.0 0.0 4 0.0 5.048340383340679E-5 0.0 0.0 0.0 5 0.0 5.048340383340679E-5 0.0 0.0 0.0 6 0.0 5.048340383340679E-5 0.0 0.0 0.0 7 0.0 5.048340383340679E-5 0.0 0.0 0.0 8 0.0 5.048340383340679E-5 0.0 0.0 0.0 9 0.0 5.048340383340679E-5 0.0 0.0 0.0 10 0.0 5.048340383340679E-5 0.0 0.0 0.0 11 0.0 5.048340383340679E-5 0.0 5.048340383340679E-5 0.0 12 0.0 1.0096680766681358E-4 0.0 5.048340383340679E-5 0.0 13 0.0 1.5145021150022035E-4 0.0 5.048340383340679E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 230 0.0 11.92189 1 GGTATCA 800 0.0 11.87399 1 GGTATAC 95 9.712641E-6 11.339241 1 GTATCAA 1910 0.0 11.228602 1 GTCCTAT 185 3.274181E-11 10.587007 1 GTTTAGT 130 2.53076E-7 10.546288 1 TAGACAG 305 0.0 10.242638 5 CTTACAC 250 0.0 9.845347 3 GTCTTAC 100 2.030275E-4 9.792981 1 GTGGTAT 390 0.0 9.792981 1 TGGTATC 345 0.0 9.651053 2 GTTCTAC 275 0.0 9.614926 1 TCTGGAC 385 0.0 9.589624 3 TAGACTG 270 0.0 9.466681 5 GTAGGAC 240 1.8189894E-12 9.466681 3 CCCGTTT 80 0.004649155 9.466681 12 TAATGCT 175 2.8263457E-8 9.196203 4 TTAGACT 165 1.12469934E-7 9.179811 4 TATACTG 270 0.0 9.116063 5 GTGTTAT 130 3.0565738E-5 9.039675 1 >>END_MODULE