FastQCFastQC Report
Thu 26 May 2016
SRR1512005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980849
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33510.16916988624574614No Hit
TATCAACGCAGAGTACTTTTTTTTT20260.10227937616648214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.012.3919891
TAGATTG951.0375043E-612.0026165
GCCCTAA600.00565746811.14005851
TAGGCAG2900.010.1573875
CAACCGT856.601334E-410.0577146
GCCTTAA1159.661462E-69.9637791
GGCCGCT1252.585608E-69.875427
CTAGAAC1752.562956E-99.7735593
CCGTGTG1856.548362E-109.7522339
TGGTATC4600.09.7086382
GAACTAT1409.87462E-79.5486221
GTCTTAC1701.531589E-89.5486221
GTGGTAT5000.09.5486221
TAGGACT2106.730261E-119.502074
GTCCTAG3050.09.3920871
GTCTTAG2550.09.3613941
TAGAACA3700.09.2452584
TTAGGCT1651.06201696E-79.2141294
GTAGGAC2501.8189894E-129.1219883
AAGGCGT1151.1120312E-49.0859546
Produced by FastQC (version 0.11.5)