FastQCFastQC Report
Thu 26 May 2016
SRR1512004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774942
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23510.1324550323334509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.012.1096171
TTAGTAC658.2783727E-411.6489523
GTCAACG600.004629892311.4435882
CGGTAGG802.805071E-411.0348891
AGGACCG700.001538523610.8168825
AGGACGT9300.010.7878065
GACCTAC1751.3096724E-1010.6495441
GGACGTG9500.010.4610646
GGCGAGG5500.010.32549719
CCCTTAT951.1900039E-410.3250431
TAGGACC3600.010.2535044
GTATCAA15150.010.1001411
CGTGAAA6850.010.0865469
AGACTGT2850.09.9629186
AACCGTG1054.2760912E-59.9154767
ACCTAAC1001.9972798E-49.808791
GACGTGG4250.09.5761237
TTAGGAC6650.09.5359363
ACGTGAA7150.09.530968
TAGGACG10350.09.5104964
Produced by FastQC (version 0.11.5)