##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512004_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1774942 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.983099166057258 31.0 28.0 34.0 19.0 34.0 2 26.08198296056998 31.0 23.0 34.0 10.0 34.0 3 28.03514650056171 31.0 27.0 34.0 16.0 34.0 4 32.483645099389165 35.0 32.0 37.0 23.0 37.0 5 32.922057734844294 35.0 35.0 37.0 26.0 37.0 6 33.191154415186524 37.0 35.0 37.0 27.0 37.0 7 33.25207415228216 37.0 35.0 37.0 27.0 37.0 8 33.28411407245983 37.0 35.0 37.0 27.0 37.0 9 34.86210253630823 39.0 35.0 39.0 27.0 39.0 10 34.75995159278444 39.0 35.0 39.0 27.0 39.0 11 34.764178209766854 39.0 35.0 39.0 27.0 39.0 12 34.653413463651205 39.0 35.0 39.0 27.0 39.0 13 34.64833780484095 39.0 35.0 39.0 27.0 39.0 14 35.80011628548989 40.0 36.0 41.0 27.0 41.0 15 35.72825252881503 40.0 36.0 41.0 27.0 41.0 16 35.669284968184876 40.0 36.0 41.0 27.0 41.0 17 35.62112564804934 40.0 36.0 41.0 27.0 41.0 18 35.55997998807848 40.0 36.0 41.0 26.0 41.0 19 35.57747858803273 40.0 36.0 41.0 26.0 41.0 20 35.47029086020839 40.0 36.0 41.0 25.0 41.0 21 35.41157513879327 40.0 36.0 41.0 25.0 41.0 22 35.30062109071733 40.0 36.0 41.0 25.0 41.0 23 35.190231568130116 40.0 36.0 41.0 25.0 41.0 24 35.084728965791555 40.0 36.0 41.0 24.0 41.0 25 34.97630176084627 40.0 36.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3188527715121587 1101 2 4.177230125358484 1101 3 2.265672297089054 1101 4 1.4689408527108014 1101 5 1.1043416133352864 1101 6 0.9601408564781693 1101 7 0.48663833944427637 1101 8 0.5012548557720322 1101 9 0.6355693266331386 1101 10 0.6746275649358964 1101 11 0.8333830301637377 1101 12 0.6224012310444991 1101 13 0.5924460541354222 1101 14 0.4216829229672783 1101 15 0.6729899803510193 1101 16 0.6959866188645876 1101 17 0.7772972710424284 1101 18 0.7042539640628931 1101 19 0.7092124373859221 1101 20 0.7985769314851936 1101 21 0.5890981999626703 1101 22 0.6211890815943519 1101 23 0.7131065497366151 1101 24 0.6858013888654853 1101 25 0.400071582877338 1102 1 1.828720789393607 1102 2 4.40910516164389 1102 3 2.8222347950732 1102 4 1.8454938681087114 1102 5 1.3447888753191464 1102 6 1.208799102780283 1102 7 1.0910774154404308 1102 8 0.9715959708467636 1102 9 1.1935864662464866 1102 10 0.9236454399334733 1102 11 1.0851868180376414 1102 12 1.1552685522461488 1102 13 1.116891935661151 1102 14 1.2065902903562176 1102 15 1.257437410054095 1102 16 1.3243789012005323 1102 17 1.2625533008706498 1102 18 1.3694557754303815 1102 19 1.2923035992285108 1102 20 1.3039294321141384 1102 21 1.3105802894860261 1102 22 1.2051293219488883 1102 23 1.2934424397128126 1102 24 1.4586458744513067 1102 25 1.293865810756138 1103 1 1.7544538384525126 1103 2 4.34431616454312 1103 3 2.7259721359775995 1103 4 2.0089286847424646 1103 5 1.7077213334405315 1103 6 1.3915807301075986 1103 7 1.1160697899432321 1103 8 1.2489044938418772 1103 9 1.2621701110184702 1103 10 1.3064099676610113 1103 11 1.378134157432342 1103 12 1.322543846559526 1103 13 1.4050475358571006 1103 14 1.3222093882984112 1103 15 1.6236904255916187 1103 16 1.5001889754836597 1103 17 1.6300133016717098 1103 18 1.567764147456053 1103 19 1.5815845379503273 1103 20 1.8742080169451043 1103 21 1.6625782467770733 1103 22 1.7201215115911523 1103 23 1.9153880044463492 1103 24 2.0123043415261037 1103 25 2.0025383118267897 1104 1 1.5624208020619896 1104 2 4.110668198919104 1104 3 2.459146092255388 1104 4 1.9636273023869464 1104 5 1.5442035658184636 1104 6 1.3901123315893997 1104 7 1.2381890239029119 1104 8 1.1971550386950085 1104 9 1.2026767857913896 1104 10 1.2444873445345124 1104 11 1.1003157339256049 1104 12 1.0743303980900123 1104 13 1.1260440889847914 1104 14 1.1948245287752002 1104 15 1.1409222110096522 1104 16 1.2899993202224138 1104 17 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-1.6543096343523445 2315 19 -1.5936436731043742 2315 20 -1.518351938769051 2315 21 -1.4774583750597259 2315 22 -1.537355584598238 2315 23 -1.4516747830918604 2315 24 -1.4953326512708287 2315 25 -1.5160176894788506 2316 1 -1.17823150567515 2316 2 -0.6469412167587407 2316 3 -0.5442211844342211 2316 4 -1.166365657085528 2316 5 -1.4832914373843806 2316 6 -1.425296276587293 2316 7 -1.6633018715449914 2316 8 -1.8847887921533264 2316 9 -1.9654518440740816 2316 10 -2.1935497688804233 2316 11 -2.1724578748677175 2316 12 -2.170055782910346 2316 13 -2.1982362083096803 2316 14 -2.4147633896171214 2316 15 -2.214643682447864 2316 16 -2.2925777938481815 2316 17 -2.484882131601637 2316 18 -2.4211449307446173 2316 19 -2.333769712714819 2316 20 -2.217839033098791 2316 21 -2.292515107307217 2316 22 -2.2336260064921944 2316 23 -2.2922204869708906 2316 24 -2.437188139571994 2316 25 -2.350337814653095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75980.0 3 2.0 4 671.0 5 3023.0 6 3386.0 7 3282.0 8 2944.0 9 2818.0 10 2780.0 11 2660.0 12 2516.0 13 2648.0 14 2718.0 15 2694.0 16 2880.0 17 3229.0 18 3643.0 19 4532.0 20 5927.0 21 7549.0 22 9094.0 23 10862.0 24 12275.0 25 14087.0 26 15892.0 27 17668.0 28 20290.0 29 24364.0 30 30526.0 31 38200.0 32 49387.0 33 64396.0 34 88507.0 35 126773.0 36 198079.0 37 276047.0 38 642613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57232067301354 18.91104047343519 13.094230684720964 25.42240816883031 2 17.332788392765913 21.61613802566086 38.280926593586244 22.77014698798698 3 18.286456684218415 24.51928006661626 29.799565281569762 27.39469796759556 4 12.533029248279663 17.129799170902483 38.27691271038716 32.060258870430694 5 13.650079833594564 37.31485310505921 35.62167101798256 13.41339604336367 6 31.629727410657594 37.0589219585327 18.30821418852796 13.003136442281743 7 27.997309207850172 31.540636257410103 22.54056752276976 17.92148701196997 8 25.16749861967165 36.244718131317114 20.85428239827825 17.733500850732984 9 26.349480715426193 15.645018259751586 20.40139903163033 37.60410199319189 10 15.027381207252077 28.81049500264798 34.45412239286962 21.70800139723033 11 33.873726578107906 22.77077222805027 24.52497039339877 18.830530800443057 12 23.017428175117836 24.784077451544896 31.50232514639915 20.696169226938117 13 28.57631404293774 21.91474425643204 25.890310781986116 23.6186309186441 14 21.278003043481444 21.761681092498286 27.657539039325812 29.302776824694455 15 23.091909482112655 30.110561359188075 23.544205951518414 23.253323207180856 16 22.707615234751334 28.073706070395538 26.961951432779212 22.256727262073916 17 22.070073275633796 28.261881233302272 27.472672346476674 22.19537314458726 18 21.928502049655986 27.6369460914559 28.867753806906403 21.56679805198171 19 23.496588597860196 27.104336509045428 27.44164558602311 21.957429307071266 20 23.72065377226144 27.078870716028234 27.683063557436068 21.517411954274255 21 23.360181915294 26.813293428557905 27.03313779091911 22.79338686522898 22 23.406343525763532 27.524519525475068 27.146078228103704 21.9230587206577 23 22.793925841540705 27.314162989054775 27.375235854504602 22.51667531489992 24 23.052566876946194 26.87810679690964 27.822139239714716 22.24718708642945 25 23.02494287117958 27.050158428247556 27.484821987947733 22.44007671262513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 192.0 2 297.0 3 402.0 4 402.0 5 402.0 6 921.5 7 1441.0 8 1441.0 9 1441.0 10 2199.5 11 2958.0 12 2958.0 13 2958.0 14 5379.5 15 7801.0 16 7801.0 17 7801.0 18 13935.0 19 20069.0 20 20069.0 21 20069.0 22 31969.0 23 43869.0 24 43869.0 25 43869.0 26 61237.0 27 78605.0 28 78605.0 29 78605.0 30 101105.0 31 123605.0 32 123605.0 33 123605.0 34 143411.5 35 163218.0 36 163218.0 37 163218.0 38 179562.0 39 195906.0 40 195906.0 41 195906.0 42 208963.5 43 222021.0 44 222021.0 45 222021.0 46 227228.5 47 232436.0 48 232436.0 49 232436.0 50 226753.0 51 221070.0 52 221070.0 53 221070.0 54 199761.5 55 178453.0 56 178453.0 57 178453.0 58 153911.0 59 129369.0 60 129369.0 61 129369.0 62 104772.0 63 80175.0 64 80175.0 65 80175.0 66 61553.0 67 42931.0 68 42931.0 69 42931.0 70 31122.0 71 19313.0 72 19313.0 73 19313.0 74 13369.5 75 7426.0 76 7426.0 77 7426.0 78 4982.5 79 2539.0 80 2539.0 81 2539.0 82 1655.0 83 771.0 84 771.0 85 771.0 86 514.5 87 258.0 88 258.0 89 258.0 90 168.5 91 79.0 92 79.0 93 79.0 94 51.0 95 23.0 96 23.0 97 23.0 98 17.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.5052976378946465 3 0.0 4 0.0 5 0.0 6 5.633986913375198E-5 7 0.0 8 1.1267973826750396E-4 9 0.0 10 1.1267973826750396E-4 11 0.0 12 0.0 13 0.0 14 5.633986913375198E-5 15 0.0 16 0.0 17 0.0 18 5.633986913375198E-4 19 6.760784296050237E-4 20 6.760784296050237E-4 21 0.002816993456687599 22 0.0016901960740125595 23 7.324182987387757E-4 24 1.6901960740125594E-4 25 3.3803921480251187E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1774942.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.03841885169066 #Duplication Level Percentage of deduplicated Percentage of total 1 82.65891802675621 62.02594512719296 2 12.013098895538436 18.028878932603913 3 3.0510109166497847 6.868291052539416 4 1.0852692148226548 3.257475436348313 5 0.46333814924526306 1.7384081056516603 6 0.23474791495301894 1.0569067420083418 7 0.12711309593414027 0.6676856013968809 8 0.07378564571318125 0.44294065506145186 9 0.05436646339124058 0.36716161062933156 >10 0.2186873597008891 2.714767457321925 >50 0.011097670431734737 0.5905013509467246 >100 0.006974959698885703 1.077487345554098 >500 0.0010611247763283864 0.5151176863780473 >1k 5.30562388164193E-4 0.6484328963670217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2351 0.1324550323334509 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 5.633986913375198E-5 0.0 13 0.0 0.0 0.0 5.633986913375198E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 810 0.0 12.109617 1 TTAGTAC 65 8.2783727E-4 11.648952 3 GTCAACG 60 0.0046298923 11.443588 2 CGGTAGG 80 2.805071E-4 11.034889 1 AGGACCG 70 0.0015385236 10.816882 5 AGGACGT 930 0.0 10.787806 5 GACCTAC 175 1.3096724E-10 10.649544 1 GGACGTG 950 0.0 10.461064 6 GGCGAGG 550 0.0 10.325497 19 CCCTTAT 95 1.1900039E-4 10.325043 1 TAGGACC 360 0.0 10.253504 4 GTATCAA 1515 0.0 10.100141 1 CGTGAAA 685 0.0 10.086546 9 AGACTGT 285 0.0 9.962918 6 AACCGTG 105 4.2760912E-5 9.915476 7 ACCTAAC 100 1.9972798E-4 9.80879 1 GACGTGG 425 0.0 9.576123 7 TTAGGAC 665 0.0 9.535936 3 ACGTGAA 715 0.0 9.53096 8 TAGGACG 1035 0.0 9.510496 4 >>END_MODULE