FastQCFastQC Report
Thu 26 May 2016
SRR1512004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774942
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28280.15932914991025057No Hit
GTCCTACAGTGGACATTTCTAAATT18430.1038343788135049No Hit
TATCAACGCAGAGTACTTTTTTTTT17870.10067934614201478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT2750.011.7514864
AGACCCG700.001498185710.85139610
TAGGACC4200.010.6364134
GGTATCA9200.010.5815841
GGCGAGG5350.010.47285919
TGGCGAG10800.010.2862218
TCTATAC1409.615178E-810.1837993
TTAGGAC7200.010.164943
GTAGGAC12200.010.1281493
GTCCTAA6600.010.1226081
GTATAAT2950.010.029471
TGTAGGA13250.09.9711562
GTACTAG1159.713305E-69.9591371
AGGACGT11300.09.9254495
GTATATA3850.09.9160251
AGTCGTC1151.0328808E-59.9077968
TAGGACG11950.09.7832644
GTCTAGC900.00106740019.5441741
GGACGTG11450.09.541816
CCTAGAC2002.6193447E-109.5048793
Produced by FastQC (version 0.11.5)