FastQCFastQC Report
Thu 26 May 2016
SRR1512003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646945
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39400.23923081827261994No Hit
GGTATCAACGCAGAGTACTTTTTTT23220.14098831472817852No Hit
TATCAACGCAGAGTACTTTTTTTTT21900.13297347513122781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA751.0725866E-513.0680371
GGTATCA8600.012.9920611
CGGTAGG600.00465706811.4345331
TAGGACC3850.011.063784
CCTACAC1701.4733814E-1010.5792883
GGCGAGG4500.010.51773919
TTAGGAC6250.010.4501093
GTGGTAT3200.010.4135921
ACTGTTC4100.010.1582888
GTATCAA18100.010.1259251
CTATACT2450.010.0452094
ATTACAC1804.2018655E-109.99154953
AATGCCG1151.0709669E-59.87752818
GTAGGAC9250.09.8238393
TGTAGGA9700.09.8010282
GATCTAC1104.856415E-59.8010281
CTAGACA2450.09.65885454
CTACACT2550.09.65127854
GGACGTG8100.09.5825386
AATCCCG1201.7737857E-59.46596619
Produced by FastQC (version 0.11.5)