FastQCFastQC Report
Thu 26 May 2016
SRR1512003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646945
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48220.29278451921588156No Hit
GGTATCAACGCAGAGTACTTTTTTT27860.1691616902810962No Hit
TATCAACGCAGAGTACTTTTTTTTT27300.16576145530057168No Hit
GTCCTACAGTGGACATTTCTAAATT16490.10012477648008888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4050.013.1414974
GGTATCA10300.012.8823181
TAGGTTG604.077323E-412.6721565
TCCAACG1457.2759576E-1212.44195118
CTTACAC2200.012.0961493
ATCCCGT951.04629E-611.99390210
TTAGGAC5800.011.30662253
CCCGTTT1105.0010203E-711.22156812
GCCGGTT600.005898182311.07770111
CCGTTTC1303.290188E-810.95596913
AGGACGT9500.010.6045945
GTATCAA21850.010.5725641
TGGACCG909.473227E-510.5601315
GTAGGAC11350.010.5508273
GGACGTG9650.010.5328046
TGTAGGA12650.010.3681292
ATTAGAC1701.546141E-910.0631843
GACGTGA5200.010.0429727
GTCCTAT2201.8189894E-129.97979451
TAGGACG10100.09.9746184
Produced by FastQC (version 0.11.5)