FastQCFastQC Report
Thu 26 May 2016
SRR1512002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863609
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29510.15834866648529816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG802.9721563E-511.8332979
TAGGACC4350.011.7516884
GGTATCA7300.011.5365961
GGACCGT600.006032286711.044416
AGGGCGT951.4145704E-510.961376
ACCGTGC700.001536060210.8190158
GTATCAA15450.010.5849821
ATTATAC2250.010.5184863
GTGCGCC1106.3052994E-610.3272411
GTAGGAC9600.010.1569133
CTTATAC2002.7284841E-119.9399693
TCCAACG1054.265904E-59.91769618
GGCGTGC1054.2671614E-59.9174298
TAGGACA4200.09.9174294
TTATACT2800.09.8047314
GTATTAG1906.0572347E-109.7926921
ACTAGGA1802.4774636E-99.7926922
CTAGAAC1752.733941E-99.7371133
AGGACGT7850.09.6475295
GGACGTG7850.09.5269356
Produced by FastQC (version 0.11.5)