##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512002_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1863609 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.91846090032834 31.0 28.0 34.0 16.0 34.0 2 26.093214832081195 31.0 23.0 34.0 10.0 34.0 3 28.007296594940247 31.0 27.0 34.0 16.0 34.0 4 32.41031621976498 35.0 32.0 37.0 22.0 37.0 5 32.84081961398555 35.0 33.0 37.0 26.0 37.0 6 33.10761860454634 37.0 35.0 37.0 26.0 37.0 7 33.15835295923125 37.0 35.0 37.0 26.0 37.0 8 33.19638346884996 37.0 35.0 37.0 27.0 37.0 9 34.76263422209272 39.0 35.0 39.0 27.0 39.0 10 34.65941246259274 39.0 35.0 39.0 27.0 39.0 11 34.6695669531538 39.0 35.0 39.0 27.0 39.0 12 34.54904918360021 39.0 35.0 39.0 27.0 39.0 13 34.541965616178075 39.0 35.0 39.0 27.0 39.0 14 35.6830837369856 40.0 36.0 41.0 27.0 41.0 15 35.612578067609675 40.0 36.0 41.0 27.0 41.0 16 35.54877015511301 40.0 36.0 41.0 26.0 41.0 17 35.50095969701799 40.0 36.0 41.0 26.0 41.0 18 35.442412008098266 40.0 36.0 41.0 26.0 41.0 19 35.4615184837592 40.0 36.0 41.0 25.0 41.0 20 35.35440213048982 40.0 36.0 41.0 25.0 41.0 21 35.28989342721569 40.0 36.0 41.0 25.0 41.0 22 35.1772249436443 40.0 36.0 41.0 24.0 41.0 23 35.06469007179081 40.0 36.0 41.0 24.0 41.0 24 34.956626631444685 40.0 36.0 41.0 23.0 41.0 25 34.841801043029946 40.0 36.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.321730972665808 1101 2 4.146009362835748 1101 3 2.307490285404185 1101 4 1.4838194076226543 1101 5 1.1395831418255753 1101 6 0.7866843800226988 1101 7 0.5573036155272391 1101 8 0.40604947419620885 1101 9 0.6666629191035582 1101 10 0.652319473592307 1101 11 0.8020176189458184 1101 12 0.5977669739788638 1101 13 0.569473605590403 1101 14 0.5479427242130441 1101 15 0.6560852080160942 1101 16 0.6794935374234257 1101 17 0.8921500656108492 1101 18 0.7158525555475777 1101 19 0.6568517670439817 1101 20 0.7548569155069842 1101 21 0.7258823483869747 1101 22 0.8124699195137808 1101 23 0.6871972899273828 1101 24 0.5201449975334143 1101 25 0.407643259442267 1102 1 2.0895528601001274 1102 2 4.736055328675231 1102 3 2.8627619293410476 1102 4 2.119897023068795 1102 5 1.4684021538637708 1102 6 1.4302858642940777 1102 7 1.2211201976532067 1102 8 1.3305766537768449 1102 9 1.278103502687479 1102 10 1.215158016049962 1102 11 1.3724116697107291 1102 12 1.2394586991152963 1102 13 1.3896183372369748 1102 14 1.5844357027791958 1102 15 1.5292728984857789 1102 16 1.5972460711131404 1102 17 1.4967350062298692 1102 18 1.6601341621245354 1102 19 1.6142333847580446 1102 20 1.731820841435166 1102 21 1.7017135808587014 1102 22 1.6271789881486711 1102 23 1.7629270229407794 1102 24 1.8052382454148344 1102 25 1.6965294484286915 1103 1 1.6136215349907914 1103 2 4.306142060431551 1103 3 2.737400853279258 1103 4 1.9322477231704696 1103 5 1.460014409011933 1103 6 1.3476842791119736 1103 7 1.1081159468171293 1103 8 1.1027465402170478 1103 9 1.0794577676113448 1103 10 0.9590653551735784 1103 11 1.1212852594009206 1103 12 1.0322494281275567 1103 13 1.0360841406694945 1103 14 1.1474362785408516 1103 15 1.2534861263036419 1103 16 1.1595220447016104 1103 17 1.1540171661936043 1103 18 1.2242256224766095 1103 19 1.1258992959927454 1103 20 1.3279730385171504 1103 21 1.3423844044428819 1103 22 1.3607342551428303 1103 23 1.526824929373383 1103 24 1.449489203996741 1103 25 1.7000863079550967 1104 1 1.48367581962059 1104 2 3.8583480889776745 1104 3 2.202719542013238 1104 4 1.6377671626108778 1104 5 1.2100002504236969 1104 6 0.8836422732918905 1104 7 0.7125659609757165 1104 8 0.8421019930809948 1104 9 0.7929650607905145 1104 10 0.6155731845716303 1104 11 0.5230692415189253 1104 12 0.7807651863350173 1104 13 0.66579910117607 1104 14 0.7001146616903284 1104 15 0.6536620542936618 1104 16 0.8358488851674579 1104 17 0.7123312699549729 1104 18 0.5603673770570623 1104 19 0.7977538300546954 1104 20 0.673575140013142 1104 21 0.6319594660478032 1104 22 0.8134795150282343 1104 23 0.7140044783470358 1104 24 0.7635104238043056 1104 25 0.8596644422749122 1105 1 1.5742253170990352 1105 2 4.1640752449690694 1105 3 2.428057328126126 1105 4 1.5559300332106218 1105 5 1.187606026913798 1105 6 0.9240111821282113 1105 7 0.8740184251292931 1105 8 0.7415962158283023 1105 9 0.7573770434056684 1105 10 0.6645013556372064 1105 11 0.7705079449177035 1105 12 0.8912712392955626 1105 13 0.7876522920251503 1105 14 0.6710151645931788 1105 15 0.8306380436595546 1105 16 0.711426918408435 1105 17 0.9308787236756473 1105 18 0.9973499482146195 1105 19 1.0977676741084679 1105 20 1.044977665849025 1105 21 0.8472022385424793 1105 22 0.9358061021228821 1105 23 0.7527097542807368 1105 24 0.8052546455250393 1105 25 0.9183409390019079 1106 1 0.5473758380363414 1106 2 2.2304830400489344 1106 3 1.133998778458178 1106 4 0.49012423417193673 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-1.1435237608183257 2315 19 -1.045670600645444 2315 20 -0.9824385738862631 2315 21 -1.0687687830558446 2315 22 -1.3314962804221935 2315 23 -1.165232826839535 2315 24 -1.0548246733961761 2315 25 -1.0747342869359358 2316 1 -1.2314506446563307 2316 2 -0.6085179186910921 2316 3 -0.7769230512959382 2316 4 -1.185798461619612 2316 5 -1.5791051775145313 2316 6 -1.7950786610311091 2316 7 -1.8257919382046417 2316 8 -1.9711389398348942 2316 9 -1.9239589902876801 2316 10 -2.06833999655899 2316 11 -2.19757310554548 2316 12 -2.2844931947815255 2316 13 -2.267852005166489 2316 14 -2.4778253249421027 2316 15 -2.23230199397031 2316 16 -2.5650164893004828 2316 17 -2.843576845569004 2316 18 -2.742474991114044 2316 19 -2.4544148827887255 2316 20 -2.631698015789439 2316 21 -2.487011934381364 2316 22 -2.7046933487984077 2316 23 -2.7426177470021003 2316 24 -2.7722970831433145 2316 25 -2.767197344054985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82634.0 3 4.0 4 673.0 5 3354.0 6 3857.0 7 3477.0 8 3234.0 9 3008.0 10 2950.0 11 2983.0 12 2845.0 13 2850.0 14 2874.0 15 2982.0 16 3081.0 17 3397.0 18 3912.0 19 4847.0 20 6233.0 21 7880.0 22 9505.0 23 11535.0 24 13127.0 25 14934.0 26 16719.0 27 18918.0 28 21770.0 29 26028.0 30 32143.0 31 40330.0 32 51688.0 33 68857.0 34 93879.0 35 133847.0 36 206534.0 37 292167.0 38 664553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18872681984258 18.585068005144855 12.7977488840202 25.42845629099237 2 17.415755907216322 21.360061278523098 38.26233202524409 22.96185078901649 3 18.74443619879492 24.080748697822344 29.52969211889404 27.6451229844887 4 12.41612376845143 16.511940004582506 38.058734423368854 33.01320180359721 5 13.497198178373255 37.405754103999286 35.597971462898066 13.499076254729397 6 32.15524286478548 37.11722791637087 17.835071627149258 12.892457591694395 7 28.34398202627268 31.49195995511934 22.01727937566303 18.146778642944952 8 25.505833036865567 36.56335636928132 20.246038734519956 17.684771859333154 9 26.263234401636826 15.685747385851862 20.02748430598908 38.02353390652224 10 14.707537900922349 29.079597705312647 34.28782539685095 21.925038996914054 11 34.72820747270484 22.64160561577026 23.93694170826606 18.693245203258837 12 22.92836104569145 24.70405541076481 31.502852797984986 20.864730745558752 13 28.689226119856688 21.664040042734285 25.842920913131458 23.80381292427757 14 21.28831745285626 21.45042227205385 27.500135489794264 29.761124785295628 15 23.22128729792569 29.88051678222202 23.36402110099275 23.534174818859537 16 22.73599236749769 28.264297929447647 26.553370368999076 22.446339334055587 17 22.208306570745258 28.12784226734256 27.264034462164545 22.39981669974764 18 22.020868156073846 27.752662178948867 28.514196946241288 21.712272718736 19 23.427515098505843 27.07863028179406 27.367426935573448 22.126427684126647 20 23.464142807392786 27.168154556688904 27.707218444291453 21.660484191626857 21 23.429431540424297 26.649638189579537 27.067122071106414 22.853808198889748 22 23.285732836937356 27.255326615746373 27.29659158838485 22.162348958931425 23 22.91178249258017 27.06565349511453 27.311094142639973 22.71146986966533 24 22.95674682833148 26.878062941314408 27.789305589316214 22.3758846410379 25 22.975799527795665 27.067879373256066 27.42632539171496 22.52999570723331 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 107.0 1 107.0 2 258.5 3 410.0 4 410.0 5 410.0 6 1030.5 7 1651.0 8 1651.0 9 1651.0 10 2654.5 11 3658.0 12 3658.0 13 3658.0 14 6337.5 15 9017.0 16 9017.0 17 9017.0 18 15481.0 19 21945.0 20 21945.0 21 21945.0 22 33365.0 23 44785.0 24 44785.0 25 44785.0 26 62390.0 27 79995.0 28 79995.0 29 79995.0 30 102578.0 31 125161.0 32 125161.0 33 125161.0 34 146014.5 35 166868.0 36 166868.0 37 166868.0 38 184389.0 39 201910.0 40 201910.0 41 201910.0 42 214494.0 43 227078.0 44 227078.0 45 227078.0 46 234096.5 47 241115.0 48 241115.0 49 241115.0 50 237237.0 51 233359.0 52 233359.0 53 233359.0 54 212693.0 55 192027.0 56 192027.0 57 192027.0 58 166513.0 59 140999.0 60 140999.0 61 140999.0 62 115151.0 63 89303.0 64 89303.0 65 89303.0 66 68977.5 67 48652.0 68 48652.0 69 48652.0 70 35547.0 71 22442.0 72 22442.0 73 22442.0 74 15520.5 75 8599.0 76 8599.0 77 8599.0 78 5855.5 79 3112.0 80 3112.0 81 3112.0 82 2046.5 83 981.0 84 981.0 85 981.0 86 639.0 87 297.0 88 297.0 89 297.0 90 200.0 91 103.0 92 103.0 93 103.0 94 64.0 95 25.0 96 25.0 97 25.0 98 17.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.3268244572761776 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.146372978452025E-4 19 7.512305424582089E-4 20 4.29274595690405E-4 21 0.00343419676552324 22 0.002307350951835927 23 0.0011805051381486137 24 0.0 25 4.8293392015170565E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1863609.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.09329059227422 #Duplication Level Percentage of deduplicated Percentage of total 1 83.43634630486392 62.6550979902877 2 11.341718389548044 17.033739096841426 3 2.901451625737714 6.536386502128456 4 1.034457814776391 3.1072336516181 5 0.4675180059655078 1.755373273954423 6 0.2299312424285713 1.035977616235881 7 0.13720002085573654 0.7211960724770133 8 0.09866343997253968 0.5927169894953059 9 0.06351132055229002 0.4292346645118972 >10 0.2670341745508472 3.3376540396161154 >50 0.014090021024505062 0.7255637365374119 >100 0.00677991506826997 1.0186070875803772 >500 7.209581420029453E-4 0.3867157288905607 >1k 5.76766513602356E-4 0.6645035498253872 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2951 0.15834866648529816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.365932446130063E-5 2 0.0 0.0 0.0 0.0 5.365932446130063E-5 3 0.0 0.0 0.0 0.0 5.365932446130063E-5 4 0.0 0.0 0.0 0.0 5.365932446130063E-5 5 0.0 1.0731864892260126E-4 0.0 0.0 5.365932446130063E-5 6 0.0 1.0731864892260126E-4 0.0 0.0 5.365932446130063E-5 7 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 5.365932446130063E-5 8 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 5.365932446130063E-5 9 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 5.365932446130063E-5 10 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 5.365932446130063E-5 11 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 5.365932446130063E-5 12 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 5.365932446130063E-5 13 0.0 1.6097797338390188E-4 0.0 5.365932446130063E-5 1.0731864892260126E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGCG 80 2.9721563E-5 11.833297 9 TAGGACC 435 0.0 11.751688 4 GGTATCA 730 0.0 11.536596 1 GGACCGT 60 0.0060322867 11.04441 6 AGGGCGT 95 1.4145704E-5 10.96137 6 ACCGTGC 70 0.0015360602 10.819015 8 GTATCAA 1545 0.0 10.584982 1 ATTATAC 225 0.0 10.518486 3 GTGCGCC 110 6.3052994E-6 10.32724 11 GTAGGAC 960 0.0 10.156913 3 CTTATAC 200 2.7284841E-11 9.939969 3 TCCAACG 105 4.265904E-5 9.917696 18 GGCGTGC 105 4.2671614E-5 9.917429 8 TAGGACA 420 0.0 9.917429 4 TTATACT 280 0.0 9.804731 4 GTATTAG 190 6.0572347E-10 9.792692 1 ACTAGGA 180 2.4774636E-9 9.792692 2 CTAGAAC 175 2.733941E-9 9.737113 3 AGGACGT 785 0.0 9.647529 5 GGACGTG 785 0.0 9.526935 6 >>END_MODULE