FastQCFastQC Report
Thu 26 May 2016
SRR1512002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863609
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34310.18410514222672245No Hit
TATCAACGCAGAGTACTTTTTTTTT20630.11069918636366319No Hit
GGTATCAACGCAGAGTACTTTTTTT20300.10892842865644027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAA250.006049717318.99063714
TAGGTCG400.005267758414.2548345
TATACCG500.001495914313.3045125
GGTATCA7950.011.2895651
TACACCG855.305584E-511.1802625
TCCAACG855.3507432E-511.17096218
TGGCGAG8700.011.132441518
TGGTATC3500.010.8608252
CGAAGGA700.001497790510.85179110
GTATCAA17450.010.779221
GTATTAA2800.010.5710991
CTACGAC909.483821E-510.5591353
GTCCTAA5100.010.4841771
GTGGTAT4100.010.479611
GTATTAG2100.010.4574321
GTCCTAC8000.010.3835481
TAGGACC4150.010.3046984
TGTAGGA10150.010.2990592
CTGTAGG10200.010.296961
GTAGGAC9550.010.2495493
Produced by FastQC (version 0.11.5)