FastQCFastQC Report
Thu 26 May 2016
SRR1512001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871231
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26760.14300746407044349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC350.002224849516.2254313
CGCTTAG450.00931206513.0791
GGTATCA8100.012.2312871
AACCGAG600.006040848311.0423087
CCAACGA700.001538162710.81724519
GAGTAAG1552.4732799E-810.1256771
TAGGACC4300.010.1251734
TGTAGGA8850.09.9755092
GCATAAC908.305116E-49.809251
GTCCTAC6900.09.809251
GTATCAA15450.09.7775041
GTAGGAC8050.09.7587753
GTCCTAA4250.09.6938471
AATCCCG1201.7761415E-59.46508819
CAGCCGA800.00465642859.4648359
TAAGAGT2950.09.3044154
GTACTGG4550.09.0546921
GTATAAT2603.6379788E-129.0546911
TCGAACT1054.6609037E-49.0143719
TAGGACT2107.1486284E-109.0141294
Produced by FastQC (version 0.11.5)