FastQCFastQC Report
Thu 26 May 2016
SRR1512001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871231
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33420.17859900781891708No Hit
TATCAACGCAGAGTACTTTTTTTTT20420.10912602452610073No Hit
GGTATCAACGCAGAGTACTTTTTTT19880.1062402236816299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG500.001495934213.3044935
GGTATCA8450.012.9941751
CGAAATC658.057879E-411.68656813
GATATAC3150.010.9118781
GTATCAA18950.010.7823171
GTCCTAC8850.010.6806941
GGTTAGA1409.001815E-810.2298861
ACGAAAT750.002658807710.1283612
GGCGAGG3200.010.08933519
GTATTAA2002.3646862E-1110.0252881
AGGACGT7600.010.0033785
GTCTAAC900.00106365869.5478931
CTTATAC2303.6379788E-129.5032093
TAGTACC800.00450360879.5032094
GGACCGT900.00111410949.4996526
TGTAGGA9750.09.4544742
GGACGTG7950.09.4399076
TAGGACG7800.09.3813734
CTGTAGG9500.09.3468861
GTCCAAT1752.4592737E-89.2750971
Produced by FastQC (version 0.11.5)