FastQCFastQC Report
Thu 26 May 2016
SRR1512000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107518
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32050.15207462047773732No Hit
CTGTAGGACGTGGAATATGGCAAGA21860.10372390650993253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.012.5773761
TCCAACG701.1289693E-412.16990218
GTACTAG802.812725E-411.0318521
CTGTGCG1303.4500772E-810.9211879
GTATCAA18950.010.3494361
GGACGTG13000.010.19310956
CGTGCGC750.002730491310.09603210
ACTGTCG750.002730491310.0960328
TAGGACG13850.09.9092374
CTCAACG908.330649E-49.8060912
GCCCTAT1201.1678094E-59.806091
AGGACGT13750.09.7747955
TAAGCTG1752.743036E-99.7354595
CTTAGAC1752.743036E-99.7354593
TTAGACT1951.7462298E-109.7077234
ATAGGAC2450.09.6581933
TTAGGAC8850.09.6254543
GTCCTAA7450.09.6086531
AGTCGGT800.0046560549.4650310
GGCGAGG7300.09.33581419
Produced by FastQC (version 0.11.5)