FastQCFastQC Report
Thu 26 May 2016
SRR1512000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107518
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36140.17148133491623796No Hit
CTGTAGGACGTGGAATATGGCAAGA24560.11653518499011634No Hit
GTCCTACAGTGGACATTTCTAAATT24260.11511170960342924No Hit
TATCAACGCAGAGTACTTTTTTTTT22990.10908566379978722No Hit
CTTTAGGACGTGAAATATGGCGAGG21800.10343921143259512No Hit
GGTATCAACGCAGAGTACTTTTTTT21760.10324941471437016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.012.0266941
AAACCGT855.329184E-511.17554956
TAGGACC3750.010.6444284
CGTGCGC909.567137E-510.55017910
GTCCTAA6850.010.4514551
GGTCTAA1303.597852E-710.2799441
AGGACGT13500.010.278355
GGACGTG13800.010.2564016
GGCGAGG6950.010.248034519
TAGGACG14300.010.2350274
GTCCTAC12150.010.2134671
GTCCTAG2250.010.182041
GACGTGA7450.09.9412417
TTAGGAC9000.09.7151533
AGGACCT9500.09.7040385
GTATCAA23100.09.6696321
TGTAGGA13200.09.6479532
TGGTATC4150.09.6184592
GTAGGAC12200.09.5818553
TCTTATA2002.6193447E-109.5039542
Produced by FastQC (version 0.11.5)