FastQCFastQC Report
Thu 26 May 2016
SRR1511999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911271
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27320.14294152948482974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5400.012.094534
ACAGCGC907.773302E-611.5686828
GTCCTAT1801.8189894E-1211.4399771
GGTATCA8450.011.0241531
GTCCTAC10550.010.5957271
CGGTTTC909.876554E-510.51698313
TGGCGAG9850.010.47427418
CGGTAGG750.002035481410.4594071
GTATCAA16600.010.3373291
GACCTAC1052.8974355E-510.2726321
GGCGAGG4900.010.23796219
GTAGGAC11550.010.1618633
CTACACT2800.010.14137654
CCTATAC1502.5878762E-810.0963043
GTGGTAT3600.010.0780741
AGGACGT9950.09.9884915
TCCTACA11950.09.8467222
CCCTAAT1002.003831E-49.8056941
GGACGTG10300.09.74097256
TGTAGGA11900.09.7232932
Produced by FastQC (version 0.11.5)