##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511999_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911271 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.440402747700354 31.0 30.0 34.0 25.0 34.0 2 26.505602816136488 31.0 25.0 34.0 10.0 34.0 3 28.460840979641297 31.0 28.0 34.0 16.0 34.0 4 32.96030756496593 35.0 33.0 37.0 27.0 37.0 5 33.41289906036349 35.0 35.0 37.0 28.0 37.0 6 33.69323711812715 37.0 35.0 37.0 29.0 37.0 7 33.760717344636106 37.0 35.0 37.0 29.0 37.0 8 33.793716851247154 37.0 35.0 37.0 29.0 37.0 9 35.41604775042367 39.0 37.0 39.0 30.0 39.0 10 35.32423083905945 39.0 37.0 39.0 30.0 39.0 11 35.342289502639865 39.0 37.0 39.0 30.0 39.0 12 35.23844080719061 39.0 35.0 39.0 29.0 39.0 13 35.23481808702167 39.0 37.0 39.0 29.0 39.0 14 36.4548046823292 40.0 37.0 41.0 29.0 41.0 15 36.39430724371374 40.0 37.0 41.0 29.0 41.0 16 36.340311237914456 40.0 37.0 41.0 29.0 41.0 17 36.29963516424411 40.0 37.0 41.0 28.0 41.0 18 36.24086851105887 40.0 37.0 41.0 28.0 41.0 19 36.26857258860727 40.0 37.0 41.0 28.0 41.0 20 36.16962115785778 40.0 37.0 41.0 27.0 41.0 21 36.12127479567262 40.0 37.0 41.0 27.0 41.0 22 36.017046771493945 40.0 37.0 41.0 27.0 41.0 23 35.924254069674056 40.0 37.0 41.0 27.0 41.0 24 35.83034797263183 40.0 37.0 41.0 27.0 41.0 25 35.73346479907873 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1305325826307282 1101 2 3.9892783010394837 1101 3 2.1671809669635955 1101 4 1.1837714963266208 1101 5 0.89017692280364 1101 6 0.5639102384739303 1101 7 0.305484152470342 1101 8 0.24221069324755717 1101 9 0.32590074590678597 1101 10 0.4001628320983812 1101 11 0.44856282891827703 1101 12 0.38313422683217624 1101 13 0.49478342254297303 1101 14 0.3389195226560915 1101 15 0.5565592913543327 1101 16 0.6422593932894003 1101 17 0.747421665240843 1101 18 0.6956228739765606 1101 19 0.6030767634857384 1101 20 0.7067873725692095 1101 21 0.5762553884495389 1101 22 0.6689376821164359 1101 23 0.6226827270760253 1101 24 0.7942609437036197 1101 25 0.546699051532741 1102 1 1.8667292744187662 1102 2 4.439028581438638 1102 3 2.79721146721959 1102 4 2.000742069854951 1102 5 1.454102949223909 1102 6 1.2682948245888426 1102 7 1.0508050227336838 1102 8 1.0891715250220102 1102 9 1.1856424001721422 1102 10 0.9399725391883464 1102 11 1.169910816718179 1102 12 1.1494277584271302 1102 13 1.247438498713926 1102 14 1.5362617570438815 1102 15 1.4209078588782305 1102 16 1.1899073274996894 1102 17 1.2982202300753016 1102 18 1.2703425246280986 1102 19 1.3699460347268442 1102 20 1.2413895245206419 1102 21 1.3486280533670723 1102 22 1.3832064628831517 1102 23 1.4863828404309984 1102 24 1.4156852319479754 1102 25 1.383192976383775 1103 1 1.3950621993554648 1103 2 4.210318951445988 1103 3 2.6575270761389724 1103 4 1.7686960027145417 1103 5 1.3449829622926046 1103 6 1.2505583220976249 1103 7 1.0525921664076208 1103 8 0.929773991737683 1103 9 0.7818217679741508 1103 10 0.9317725881959475 1103 11 0.9238613190460399 1103 12 0.9868787099912879 1103 13 1.0768368487915225 1103 14 0.9727871184749048 1103 15 1.230101683921113 1103 16 1.0272628776099566 1103 17 0.997256740734457 1103 18 1.1231397148592563 1103 19 1.0678200852046729 1103 20 1.280534178608697 1103 21 1.2768825661498866 1103 22 1.2017878563324658 1103 23 1.3146641440330527 1103 24 1.4360681446327703 1103 25 1.5110753581529508 1104 1 1.4689557567784348 1104 2 3.9929277282900593 1104 3 2.2106381872500833 1104 4 1.5998631362346174 1104 5 1.2365005471043062 1104 6 0.8857335477434418 1104 7 0.8122933802264853 1104 8 0.9757322861665685 1104 9 0.8840676441023803 1104 10 0.7779667992136865 1104 11 0.7608498033286395 1104 12 1.054061405291634 1104 13 0.948363770671385 1104 14 0.907713978107644 1104 15 0.8270951479734023 1104 16 0.9493749224278858 1104 17 0.8544886112604431 1104 18 0.9628110500599973 1104 19 0.9904929765834467 1104 20 0.8344034596544461 1104 21 0.810909954883158 1104 22 0.7552201625883015 1104 23 0.912215342290132 1104 24 1.0596386394973862 1104 25 1.0139623796282038 1105 1 1.328528064665992 1105 2 4.056399395274006 1105 3 2.3980707654593623 1105 4 1.4621957715541214 1105 5 1.1257331549262943 1105 6 0.966740168298891 1105 7 0.6605070993711024 1105 8 0.5237471771283495 1105 9 0.4407442521781917 1105 10 0.44874222912675066 1105 11 0.5212618430096683 1105 12 0.5785606332460276 1105 13 0.5262663448617957 1105 14 0.48261297762115163 1105 15 0.4184080485381614 1105 16 0.5594231488381851 1105 17 0.6433975944869559 1105 18 0.635220466901167 1105 19 0.72828302248314 1105 20 0.6938329919851824 1105 21 0.6861505581363261 1105 22 0.7161789797721312 1105 23 0.4872941332455696 1105 24 0.64349548320574 1105 25 0.5721830789111593 1106 1 0.5723252599194986 1106 2 2.284015360754047 1106 3 1.2837234083935556 1106 4 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-0.9275338976031264 2315 11 -1.0423989607335287 2315 12 -0.9023353637687777 2315 13 -1.1129944356510393 2315 14 -1.0110789264593905 2315 15 -1.4640399014964345 2315 16 -1.2102411495201864 2315 17 -1.1869535178496946 2315 18 -1.1811197171034422 2315 19 -1.1359679605774389 2315 20 -1.1901839222815127 2315 21 -1.09109539061145 2315 22 -1.3626021394284464 2315 23 -1.192170955585425 2315 24 -1.3042133305685795 2315 25 -1.2317105680869815 2316 1 -0.8001400011478417 2316 2 -0.16046421704467662 2316 3 -0.24654010944070848 2316 4 -0.6636525663730666 2316 5 -1.0306591047759284 2316 6 -0.8988106718744007 2316 7 -1.191214236120615 2316 8 -1.2153810384021853 2316 9 -1.3255356382410994 2316 10 -1.4160867200500888 2316 11 -1.4539899128846656 2316 12 -1.4179207281780748 2316 13 -1.5725525115408914 2316 14 -1.6705335346532024 2316 15 -1.3936262964148156 2316 16 -1.4890885006308139 2316 17 -1.7115239697471978 2316 18 -1.6795151689705605 2316 19 -1.465172716340362 2316 20 -1.6356378820221948 2316 21 -1.7069987471292123 2316 22 -1.7044348861033072 2316 23 -1.695239993615452 2316 24 -1.8003298187311856 2316 25 -1.6628683417358516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67936.0 3 3.0 4 572.0 5 2746.0 6 3124.0 7 2916.0 8 2617.0 9 2651.0 10 2550.0 11 2462.0 12 2440.0 13 2373.0 14 2380.0 15 2567.0 16 2763.0 17 2857.0 18 3332.0 19 4133.0 20 5201.0 21 6627.0 22 8253.0 23 9853.0 24 11543.0 25 13073.0 26 14520.0 27 16948.0 28 19335.0 29 23277.0 30 29312.0 31 36892.0 32 47738.0 33 63408.0 34 88053.0 35 129333.0 36 212958.0 37 295401.0 38 769124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.043503511537615 18.67704789116771 12.968385958872394 25.31106263842229 2 17.29917211310082 21.372804939786594 38.693874807717116 22.63414813939547 3 18.381956300283946 24.40794633518742 29.801791582669335 27.408305781859298 4 12.368575675558306 16.85658391719437 38.46089853296576 32.31394187428157 5 13.482860358368857 37.413584991348685 35.764577603071466 13.338977047210992 6 31.637951917859898 37.25128461636262 18.236294068188133 12.874469397589353 7 28.188362613151146 31.452996461516967 22.419583617393872 17.93905730793802 8 25.16111006759376 36.551750118115116 20.647307472357397 17.63983234193372 9 26.265087473205 15.75422846890891 20.327258667138253 37.65342539074783 10 14.84860074787929 28.997405391490794 34.39093671174836 21.763057148881558 11 34.12006983834318 22.783529912817176 24.44373403876269 18.65266621007696 12 22.89361372615396 24.687132280037734 31.719049784148872 20.700204209659436 13 28.636127477474417 21.825005454485524 25.914430763612277 23.62443630442779 14 21.228282122210825 21.71926430108551 27.75885784904391 29.29359572765976 15 23.15328386189086 30.023528845464615 23.57195813675821 23.251229155886318 16 22.687572824575895 28.190717067333726 26.845852838242195 22.275857269848178 17 22.128154510794126 28.243927731860108 27.468004275688795 22.15991348165697 18 21.845008410659954 27.769723193800967 28.77565382089872 21.609614574640357 19 23.418268576750418 27.133670981446794 27.471720226447477 21.976340215355318 20 23.465019447883126 27.210398155773515 27.81947216027944 21.50511023606392 21 23.33654072047438 26.75023662982619 27.130727972235434 22.782494677463998 22 23.340184048437767 27.294169846110055 27.35235209047967 22.01329401497251 23 22.92866358945488 27.203867199231503 27.278634864858358 22.588834346455258 24 22.980321984858236 26.932249237418056 27.866654109571122 22.22077466815259 25 23.121468638343046 26.984089611760158 27.467486227722237 22.42695552217456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 150.0 2 276.0 3 402.0 4 402.0 5 402.0 6 938.5 7 1475.0 8 1475.0 9 1475.0 10 2309.0 11 3143.0 12 3143.0 13 3143.0 14 5645.0 15 8147.0 16 8147.0 17 8147.0 18 15054.0 19 21961.0 20 21961.0 21 21961.0 22 34302.5 23 46644.0 24 46644.0 25 46644.0 26 66077.0 27 85510.0 28 85510.0 29 85510.0 30 110056.0 31 134602.0 32 134602.0 33 134602.0 34 155207.5 35 175813.0 36 175813.0 37 175813.0 38 193911.5 39 212010.0 40 212010.0 41 212010.0 42 225500.0 43 238990.0 44 238990.0 45 238990.0 46 244135.0 47 249280.0 48 249280.0 49 249280.0 50 243723.0 51 238166.0 52 238166.0 53 238166.0 54 214199.0 55 190232.0 56 190232.0 57 190232.0 58 163826.0 59 137420.0 60 137420.0 61 137420.0 62 111943.0 63 86466.0 64 86466.0 65 86466.0 66 66417.0 67 46368.0 68 46368.0 69 46368.0 70 33817.0 71 21266.0 72 21266.0 73 21266.0 74 14963.5 75 8661.0 76 8661.0 77 8661.0 78 5911.0 79 3161.0 80 3161.0 81 3161.0 82 2076.5 83 992.0 84 992.0 85 992.0 86 642.0 87 292.0 88 292.0 89 292.0 90 180.5 91 69.0 92 69.0 93 69.0 94 51.5 95 34.0 96 34.0 97 34.0 98 25.5 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.470622428739828 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.139272243444284E-4 19 5.755332446314521E-4 20 4.7089083651664253E-4 21 0.0030346298353294745 22 0.0018312421420091658 23 8.894604689758804E-4 24 5.232120405740473E-5 25 2.0928481622961893E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1911271.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.17846186231907 #Duplication Level Percentage of deduplicated Percentage of total 1 84.03036612700298 64.01304041282604 2 11.176666043434645 17.02842455875325 3 2.6947889532814773 6.158546325136552 4 0.9269806109637133 2.8246382847763383 5 0.41432979531408354 1.5781503255378186 6 0.22119959610982465 1.0110386997727594 7 0.13354668288108928 0.7121366622087396 8 0.09325259035496006 0.568307111833423 9 0.05759494799942416 0.3948745094672744 >10 0.23230926744324915 2.906106221202509 >50 0.011065740098902512 0.5937515629669967 >100 0.0064449404360348355 1.0553206051560065 >500 9.005314684083766E-4 0.48585411325918193 >1k 5.541732113282257E-4 0.6698106071031547 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2732 0.14294152948482974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.0464240811480946E-4 0.0 0.0 0.0 5 0.0 1.0464240811480946E-4 0.0 0.0 0.0 6 0.0 1.0464240811480946E-4 0.0 0.0 0.0 7 0.0 1.0464240811480946E-4 0.0 0.0 0.0 8 0.0 1.0464240811480946E-4 0.0 0.0 0.0 9 0.0 1.0464240811480946E-4 0.0 0.0 0.0 10 0.0 1.0464240811480946E-4 0.0 0.0 0.0 11 0.0 1.0464240811480946E-4 0.0 0.0 0.0 12 0.0 1.0464240811480946E-4 0.0 0.0 0.0 13 0.0 1.0464240811480946E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 540 0.0 12.09453 4 ACAGCGC 90 7.773302E-6 11.568682 8 GTCCTAT 180 1.8189894E-12 11.439977 1 GGTATCA 845 0.0 11.024153 1 GTCCTAC 1055 0.0 10.595727 1 CGGTTTC 90 9.876554E-5 10.516983 13 TGGCGAG 985 0.0 10.474274 18 CGGTAGG 75 0.0020354814 10.459407 1 GTATCAA 1660 0.0 10.337329 1 GACCTAC 105 2.8974355E-5 10.272632 1 GGCGAGG 490 0.0 10.237962 19 GTAGGAC 1155 0.0 10.161863 3 CTACACT 280 0.0 10.1413765 4 CCTATAC 150 2.5878762E-8 10.096304 3 GTGGTAT 360 0.0 10.078074 1 AGGACGT 995 0.0 9.988491 5 TCCTACA 1195 0.0 9.846722 2 CCCTAAT 100 2.003831E-4 9.805694 1 GGACGTG 1030 0.0 9.7409725 6 TGTAGGA 1190 0.0 9.723293 2 >>END_MODULE