FastQCFastQC Report
Thu 26 May 2016
SRR1511999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911271
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31920.1670092833512359No Hit
TATCAACGCAGAGTACTTTTTTTTT19630.10270652356468549No Hit
GGTATCAACGCAGAGTACTTTTTTT19470.10186938429976701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1051.378794E-913.56602918
GGTATCA9450.013.0288831
CCAACGA1157.1049726E-811.56182519
ATGTCGA700.001497941910.851686517
TAGGACC4700.010.7169154
GTAGGAC10600.010.6692253
AGGACGT10100.010.6328295
TGTAGGA12350.010.54254152
GTACTAG1105.7189864E-610.4120881
TAGGACG11200.010.2673674
GTCCTAA6800.010.246211
TGGCGAG9600.010.18865218
GTCCTAG3000.010.1807091
ACTGTTC4200.010.1734568
TAGGACA4400.010.1516574
GGACGTG10350.010.0968276
GGCGAGG5100.010.05587219
AAAGGCG1356.409082E-79.8556665
GTCCTAC11150.09.8440151
GTATCAA19600.09.8365911
Produced by FastQC (version 0.11.5)