FastQCFastQC Report
Thu 26 May 2016
SRR1511998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965473
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24850.12643267040554615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCACG802.9781604E-511.83103757
GTATCAA14500.011.7027691
ACCGATC658.2786486E-411.6490218
GGTATCA6200.010.7579061
GTCTAAG2200.010.7003771
AGGACGT7850.010.3691125
GGCGAGG3600.010.25408819
TCTACAC3600.010.2535663
GTATAAT2600.010.1859361
TACACCG750.0027308310.09581855
GGACGTG7700.09.956516
TAGGACG8250.09.8663674
GTCCTAA4000.09.808681
GTATTAA2700.09.8086791
TTAGGAC5700.09.7969293
GTCCTAC6450.09.7326431
TAATACT2350.09.6662084
TAGGACC3450.09.6020014
AATCCCG900.00115167519.46531219
GACGTGG4350.09.3560397
Produced by FastQC (version 0.11.5)