FastQCFastQC Report
Thu 26 May 2016
SRR1511998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965473
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30530.15533156649824242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.011.6174941
CTAGTAC855.2986947E-511.1817583
AGGACCG1408.292773E-910.862285
TAGACTG2750.010.7141575
GTCCTAA5350.010.7102511
CTTAGAC1803.45608E-1110.5605493
TAGTACC1106.0126476E-610.368544
TTACACC1752.3464963E-1010.3191664
CCTACAC2950.010.3099593
GTCCTAC7900.010.2752891
TTAGTAC856.565979E-410.0635823
GTATCAA19250.010.021271
GCGTGCA951.6538179E-49.9942979
TTAGACA1252.554796E-69.8846754
TCCTACA9150.09.8680542
GTCTAAG2053.6379788E-119.78291
GTATTAA2159.094947E-129.7720661
TAGGACC3750.09.6312214
TAGGACG8050.09.5635294
CTTGCAC1901.0422809E-99.5044963
Produced by FastQC (version 0.11.5)