FastQCFastQC Report
Thu 26 May 2016
SRR1511997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329417
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36310.15587591229908598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4150.012.772474
ACCGGGC658.276114E-411.6496168
GTAGGAC9250.011.2560483
GGTATCA10650.011.2325161
ACCGTCA600.00603933511.0428658
TAGGACG9750.010.9700554
TTAGGAC6300.010.81753
GTATCAA21000.010.6925931
GCTCGAA909.878382E-510.51701411
GGACGTG9550.010.5060016
AGGACGT10400.010.4664525
TATGGCG10000.010.41184416
TGGCGAG10100.010.21504118
ATGGCGA10600.010.17967717
GTATTAC1552.4898327E-810.1217381
GGCGAGG5200.010.01138919
TGTAGGA9950.09.9532552
TCTATAC1752.743036E-99.735753
GTCCTAC10550.09.5730781
GACGTGG4750.09.5649477
Produced by FastQC (version 0.11.5)