##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511997_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2329417 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.4850260816333 31.0 30.0 34.0 25.0 34.0 2 26.54759667333071 31.0 25.0 34.0 10.0 34.0 3 28.50359381768056 31.0 28.0 34.0 16.0 34.0 4 33.014757340570625 35.0 33.0 37.0 27.0 37.0 5 33.45918785687578 35.0 35.0 37.0 28.0 37.0 6 33.73825382059116 37.0 35.0 37.0 29.0 37.0 7 33.80596861789881 37.0 35.0 37.0 29.0 37.0 8 33.84706473765753 37.0 35.0 37.0 30.0 37.0 9 35.46465102641562 39.0 37.0 39.0 30.0 39.0 10 35.38127093603249 39.0 37.0 39.0 30.0 39.0 11 35.38831518787748 39.0 37.0 39.0 30.0 39.0 12 35.290146847902285 39.0 35.0 39.0 29.0 39.0 13 35.28368772100487 39.0 35.0 39.0 30.0 39.0 14 36.49725575111712 40.0 37.0 41.0 30.0 41.0 15 36.43079877926537 40.0 37.0 41.0 29.0 41.0 16 36.375466908672855 40.0 37.0 41.0 29.0 41.0 17 36.33644641556235 40.0 37.0 41.0 29.0 41.0 18 36.28135408988601 40.0 37.0 41.0 29.0 41.0 19 36.30114487874005 40.0 37.0 41.0 29.0 41.0 20 36.205107544076476 40.0 37.0 41.0 28.0 41.0 21 36.1509545092184 40.0 37.0 41.0 28.0 41.0 22 36.04909769268448 40.0 37.0 41.0 27.0 41.0 23 35.95237434946169 40.0 37.0 41.0 27.0 41.0 24 35.85248369012504 40.0 36.0 41.0 27.0 41.0 25 35.75211136520426 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.104189849642836 1101 2 3.950430616362425 1101 3 2.1788075353647756 1101 4 1.2616413505954398 1101 5 0.7330729384126613 1101 6 0.4708063341379969 1101 7 0.29457799980586685 1101 8 0.1314040050632883 1101 9 0.2887627280352092 1101 10 0.4642224675802993 1101 11 0.4134130151311126 1101 12 0.2412448861891292 1101 13 0.38507407040906827 1101 14 0.12692744217886798 1101 15 0.24727999535759437 1101 16 0.5107038656621015 1101 17 0.5515272338645616 1101 18 0.6165259325659775 1101 19 0.5526216263103549 1101 20 0.4005357940748766 1101 21 0.4717029309584717 1101 22 0.3986124048364843 1101 23 0.5803741344084585 1101 24 0.3671470332376714 1101 25 0.29462418686863145 1102 1 1.566952963642489 1102 2 4.305196243769824 1102 3 2.454386751251228 1102 4 1.5737178996705339 1102 5 1.164003796923609 1102 6 1.0617887469826357 1102 7 0.802943521103515 1102 8 0.8046281739707197 1102 9 0.8973564120934228 1102 10 0.7068172261337224 1102 11 0.8316395085387711 1102 12 0.8215319184253218 1102 13 0.838267210252873 1102 14 0.8819713397293683 1102 15 0.9465157907854973 1102 16 0.8815266315628207 1102 17 0.893821616076707 1102 18 0.9792785963628603 1102 19 0.993078916465592 1102 20 1.0030435747867514 1102 21 0.8854485649843298 1102 22 0.830256690652206 1102 23 0.9394176461053121 1102 24 1.112217016102015 1102 25 1.0469155278038187 1103 1 1.2025060500193767 1103 2 4.00048223446025 1103 3 2.348277655993016 1103 4 1.5699986992986084 1103 5 1.1712840159088955 1103 6 0.9477821380716023 1103 7 0.6955709008226805 1103 8 0.6569327085787648 1103 9 0.6233682448605862 1103 10 0.4600093147309394 1103 11 0.6754562414681686 1103 12 0.542680626639779 1103 13 0.6252716142645198 1103 14 0.5797284381357812 1103 15 0.8235950610660439 1103 16 0.5516675097802235 1103 17 0.6476039926997572 1103 18 0.7848693570049221 1103 19 0.6883340424226461 1103 20 0.7556550205873123 1103 21 0.9548556341657033 1103 22 0.8695474962410401 1103 23 0.9707318471620425 1103 24 0.9838351576206747 1103 25 1.087680124411392 1104 1 1.4523116291049014 1104 2 3.9945738748076316 1104 3 2.3475257358332655 1104 4 1.7735438397522714 1104 5 1.2500228760527818 1104 6 1.0844450295547148 1104 7 0.9845918484166134 1104 8 0.8836452592045632 1104 9 0.8957775537111132 1104 10 0.9178183660711525 1104 11 1.0110050278723506 1104 12 0.978050502170916 1104 13 0.9318742794152968 1104 14 0.9664335589011372 1104 15 1.0236820752033466 1104 16 1.1128725433860112 1104 17 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-0.9771618288957598 2315 11 -1.1206079161596492 2315 12 -1.065077981498824 2315 13 -1.097526667747566 2315 14 -1.2186491794905834 2315 15 -1.4698765848537363 2315 16 -1.2995969584442761 2315 17 -1.337039427746241 2315 18 -1.2159236351573668 2315 19 -1.2124210957686188 2315 20 -1.2148795454266192 2315 21 -1.1986019378417723 2315 22 -1.387832814840955 2315 23 -1.4817240505351705 2315 24 -1.4407209075075613 2315 25 -1.3646932710412756 2316 1 -0.8187381539797549 2316 2 -0.21760270152778105 2316 3 -0.2764685824950668 2316 4 -0.5663546158694217 2316 5 -0.9696668422949486 2316 6 -1.008848983139714 2316 7 -1.237788436784399 2316 8 -1.37100571154226 2316 9 -1.2969567645926645 2316 10 -1.412888202049352 2316 11 -1.5340466560611787 2316 12 -1.4139421719477028 2316 13 -1.5879200899952792 2316 14 -1.6738568854038647 2316 15 -1.4905078291770764 2316 16 -1.6805680851711742 2316 17 -1.8292924035099034 2316 18 -1.8216184580456485 2316 19 -1.6906069555133456 2316 20 -1.584146718809663 2316 21 -1.635498539017732 2316 22 -1.7486662676828644 2316 23 -1.6426177357017622 2316 24 -1.7161454019862674 2316 25 -1.6518346257905492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79269.0 3 2.0 4 657.0 5 3219.0 6 3647.0 7 3407.0 8 3266.0 9 3108.0 10 2967.0 11 2853.0 12 2833.0 13 2860.0 14 2954.0 15 3037.0 16 3189.0 17 3484.0 18 4064.0 19 4903.0 20 6246.0 21 7969.0 22 9844.0 23 11746.0 24 13930.0 25 15494.0 26 17689.0 27 20248.0 28 23631.0 29 28607.0 30 35838.0 31 45920.0 32 60017.0 33 80316.0 34 110890.0 35 162763.0 36 264884.0 37 366750.0 38 916916.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32830918637582 18.32261033554748 12.80483485782065 25.544245620256056 2 17.67670074410612 21.18461054058429 37.83213940738342 23.30654930792616 3 18.746235646086554 23.894261954815306 29.41220914932792 27.947293249770222 4 12.778733906380868 16.494384646458748 37.40541088177857 33.32147056538181 5 13.977574646360011 36.95323765560224 35.175883064303214 13.893304633734536 6 32.604137689446624 36.30120167458281 17.76479598319922 13.32986465277134 7 28.60788772469678 31.23245000787751 21.885561923863353 18.274100343562356 8 25.9434553553337 35.651339219787275 20.231508669984237 18.173696754894788 9 26.46284456582913 15.660442076279171 19.92653097320059 37.950182384691104 10 15.357418064192082 28.639980424269613 33.56782711539164 22.434774396146672 11 34.57749299502837 22.54431044334269 23.831971690770697 19.046224870858243 12 23.31995516474723 24.487242945337826 30.83672867502899 21.356073214885956 13 28.815149885142933 21.484903733423426 25.593828842152348 24.10611753928129 14 21.800347468916044 21.133399472915325 27.06848966930352 29.997763388865113 15 23.52670217483602 29.262901404085227 23.33777078127274 23.87262563980601 16 23.355157105833776 27.65803632411028 26.04677479386473 22.940031776191212 17 22.618535023999566 27.706503386898955 26.873634046630553 22.80132754247093 18 22.605072031362557 27.200457456463074 27.97748091570283 22.21698959647154 19 23.904718893518595 26.6945765479724 26.961426151432853 22.439278407076152 20 24.07190852252336 26.640033244555262 27.171113297650386 22.116944935270997 21 23.916546962725352 26.254487287913662 26.63411978751227 23.194845961848714 22 23.90617936696487 26.700398088590916 26.758224609885627 22.63519793455858 23 23.501588391860565 26.509487421653645 26.863355370481667 23.125568816004122 24 23.500654241234713 26.435595874674167 27.233607050007386 22.830142834083738 25 23.56398248312556 26.486437987972067 27.02850634774198 22.921073181160388 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 343.5 3 502.0 4 502.0 5 502.0 6 1219.0 7 1936.0 8 1936.0 9 1936.0 10 2998.5 11 4061.0 12 4061.0 13 4061.0 14 6853.0 15 9645.0 16 9645.0 17 9645.0 18 17111.0 19 24577.0 20 24577.0 21 24577.0 22 37736.5 23 50896.0 24 50896.0 25 50896.0 26 70902.5 27 90909.0 28 90909.0 29 90909.0 30 116927.0 31 142945.0 32 142945.0 33 142945.0 34 167617.0 35 192289.0 36 192289.0 37 192289.0 38 215643.5 39 238998.0 40 238998.0 41 238998.0 42 259891.0 43 280784.0 44 280784.0 45 280784.0 46 292487.0 47 304190.0 48 304190.0 49 304190.0 50 303779.5 51 303369.0 52 303369.0 53 303369.0 54 279000.5 55 254632.0 56 254632.0 57 254632.0 58 222462.0 59 190292.0 60 190292.0 61 190292.0 62 156520.5 63 122749.0 64 122749.0 65 122749.0 66 94818.0 67 66887.0 68 66887.0 69 66887.0 70 49128.0 71 31369.0 72 31369.0 73 31369.0 74 21740.5 75 12112.0 76 12112.0 77 12112.0 78 8159.5 79 4207.0 80 4207.0 81 4207.0 82 2760.5 83 1314.0 84 1314.0 85 1314.0 86 850.5 87 387.0 88 387.0 89 387.0 90 255.0 91 123.0 92 123.0 93 123.0 94 83.5 95 44.0 96 44.0 97 44.0 98 29.5 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4692371524720564 3 0.0 4 0.0 5 0.0 6 4.292919644700799E-5 7 0.0 8 4.292919644700799E-5 9 0.0 10 8.585839289401598E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 6.439379467051198E-4 19 6.439379467051198E-4 20 5.580795538111038E-4 21 0.002532822590373471 22 0.0015454510720922874 23 7.297963395991358E-4 24 4.292919644700799E-5 25 2.575751786820479E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2329417.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.59462712231883 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04371666232815 56.50656331261593 2 13.018380377838893 18.380554170200973 3 3.7021073172494754 7.840466568841044 4 1.3930469497339375 3.9336651992140377 5 0.6510760643811514 2.2981235996627114 6 0.34604854871840074 1.465750095779705 7 0.21133584947413933 1.0443422843843757 8 0.14694023853760813 0.8298553079061624 9 0.09506356124213751 0.6039878992927574 >10 0.3699047782271535 4.28948046004309 >50 0.01406396753970287 0.6828731507814145 >100 0.006839626850661264 0.9436018087233886 >500 7.995313508909465E-4 0.40438964632378827 >1k 6.765265276737814E-4 0.7763464962305452 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3631 0.15587591229908598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.292919644700799E-5 0.0 10 0.0 0.0 0.0 4.292919644700799E-5 0.0 11 0.0 0.0 0.0 4.292919644700799E-5 0.0 12 0.0 0.0 0.0 4.292919644700799E-5 0.0 13 0.0 0.0 0.0 4.292919644700799E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 415 0.0 12.77247 4 ACCGGGC 65 8.276114E-4 11.649616 8 GTAGGAC 925 0.0 11.256048 3 GGTATCA 1065 0.0 11.232516 1 ACCGTCA 60 0.006039335 11.042865 8 TAGGACG 975 0.0 10.970055 4 TTAGGAC 630 0.0 10.8175 3 GTATCAA 2100 0.0 10.692593 1 GCTCGAA 90 9.878382E-5 10.517014 11 GGACGTG 955 0.0 10.506001 6 AGGACGT 1040 0.0 10.466452 5 TATGGCG 1000 0.0 10.411844 16 TGGCGAG 1010 0.0 10.215041 18 ATGGCGA 1060 0.0 10.179677 17 GTATTAC 155 2.4898327E-8 10.121738 1 GGCGAGG 520 0.0 10.011389 19 TGTAGGA 995 0.0 9.953255 2 TCTATAC 175 2.743036E-9 9.73575 3 GTCCTAC 1055 0.0 9.573078 1 GACGTGG 475 0.0 9.564947 7 >>END_MODULE