FastQCFastQC Report
Thu 26 May 2016
SRR1511997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329417
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43560.18699957972316678No Hit
TATCAACGCAGAGTACTTTTTTTTT27540.11822700701506No Hit
GGTATCAACGCAGAGTACTTTTTTT26600.11419166254904124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG802.8561419E-511.8797275
GGTATCA11050.011.576681
GGACGTG8950.011.0389946
AGGACGT8850.010.953515
GTATCAA23200.010.3694111
GTTAATC1201.5166879E-610.2957643
TAGGACG9750.010.2348414
GTGGTAT4950.010.2214421
GTCCTAC9500.09.8479081
GGCGAGG4350.09.82368919
TGTAGGA12150.09.6993322
CTTACAC3250.09.6499933
GACGTGG5350.09.5839547
GGTCTAC2600.09.5464411
ATCGTGC800.00453569139.49521358
TGGCGAG10150.09.4484418
GTAGGAC11300.09.4196773
TAGGACC5200.09.3210164
TGGTATC5200.09.3210162
GTCCTAA5850.09.3016611
Produced by FastQC (version 0.11.5)